User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/14

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Purpose

  • To harvest cells grown on 2013/02/13 and store pellet in -80°C freezer for future use.

Procedure

  • Cells were taken off the orbital shaker and were centrifuged at 4700rpm, at 4°C, for 30 minutes.
  • The supernatent was poured out, leaving the pellet alone.
  • The pellet was placed in -80°C freezer.