User:Keyun Wang/Notebook/Experimental Biological Chemistry I/2013/02/19

Experimental Biological Chemistry I

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Purpose To make starter cultures of DH5α-T1 cells and autoclaved LB media for future use. Procedure One colony of DH5α-T1 cell was inoculated with a sterile wooden stick and dipped into 3mL of sterile LB media inside a 15mL falcon tube. This process was repeated three more times to obtain a total of four cell culture-containing falcon tubes. The culture were placed in orbital shaker at 230rpm, at 37°C, for 15 hours. 1.25g of LB media was weighted out and placed in a 250mL Erlenmeyer flask. 50mL of distilled water was weighted out and poured into the Erlenmeyer flask. This process was repeated five more times to obtain a total of six LB-containing flasks. The flasks were placed in autoclave under liquid cycle.