Difference between revisions of "User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01"

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(12/01/12)
(12/01/12)
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| bgcolor=#cfcfcf | Volume
 
| bgcolor=#cfcfcf | Volume
 
| rowspan="7" | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
 
| rowspan="7" | <u>Expected:</u><br>1. MV2 = 4529<br>2. KAH3/MV2-1 = 5280<br>3. KAH3/MV2-2 = 5280<br>
| rowspan="7" | [[Image:GelImage.jpg|400px|Hover name]]<br>15 μL/lane; 1% agarose; [http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
+
| rowspan="7" | [[Image:KAH120112_gel1.png|200px|Hover name]]<br>15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid[http://openwetware.org/wiki/Image:KAH_Fermentas_GeneRuler_1kbplus.jpg Ladder]
 
|-
 
|-
 
| DNA(plasmid) || 2.0 μL
 
| DNA(plasmid) || 2.0 μL

Revision as of 11:55, 2 December 2012

Owwnotebook icon.pngKarmella's BioBrick Cloning <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)



Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
Hover name
15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmidLadder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15 μL --> 37°C/ ~15 min.