User:Carly M. Montanero/Notebook/CHEM-571/2013/11/20: Difference between revisions

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(Autocreate 2013/11/20 Entry for User:Carly_M._Montanero/Notebook/CHEM-571)
 
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==Entry title==
==Objective==
* Insert content here...
To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.


==Procedure==
'''Preparing Invertase-AuNP Samples for UV-Vis'''
# Centrifuge 1mL of the sample for 30 min at max rpm.
# Take UV-Vis measurements of the samples.
'''Creating the Gold Stock Solution'''
# Add 0.0562 g of HAuCl<sub>4</sub>·3H<sub>2</sub>O to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of gold stock was 2.85 mM.
'''Creating the BSA Stock Solution'''
# Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
# The final concentration of BSA stock was 21.8 mM.
Solutions with the following Au:protein ratio were synthesized:
:''Gold to BSA Ratio''
::*105
::*110
::*115
::*120
::*125
::*130
::*135
::*140
::*145
::*150


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Revision as of 10:35, 20 November 2013

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Objective

To synthesize BSA-AuNPs and run UV-Vis on the invertase-AuNP solutions created yesterday.

Procedure

Preparing Invertase-AuNP Samples for UV-Vis

  1. Centrifuge 1mL of the sample for 30 min at max rpm.
  2. Take UV-Vis measurements of the samples.


Creating the Gold Stock Solution

  1. Add 0.0562 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.85 mM.


Creating the BSA Stock Solution

  1. Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of BSA stock was 21.8 mM.

Solutions with the following Au:protein ratio were synthesized:

Gold to BSA Ratio
  • 105
  • 110
  • 115
  • 120
  • 125
  • 130
  • 135
  • 140
  • 145
  • 150


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