User:Carly M. Montanero/Notebook/CHEM-571/2013/11/19

Biomaterials Design Lab: Fall 2013

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Objective To synthesize invertase-AuNPs and BSA-AuNPs. Procedure Creating the Gold Stock Solution Add 0.0345 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water. The final concentration of gold stock was 3.59 mM. Creating the Invertase Stock Solution Add 0.1015 g of invertase to a 50 mL volumetric flask and fill with distilled water. The final concentration of invertase stock was 30.07μM Creating the BSA Stock Solution Add 0.0432 g of BSA to a 50 mL volumetric flask and fill with distilled water. The final concentration of BSA stock was 21.8 mM. Solutions with the following Au:protein ratio were synthesized: Gold to Invertase Ratio 30 50 70 90 110 130 150 170 190 210 Gold to BSA Ratio 105 110 115 120 125 130 135 140 145 150 As seen below, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil. The samples were heated at 80°C for 4 hours. Figures Notes The BSA stock concentration was calculated incorrectly. We will remake the BSA-AuNP solutions tomorrow.