User:Carly M. Montanero/Notebook/CHEM-571/2014/01/28

Biomaterials Design Lab: Spring 2014

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Objective To synthesize lysozyme-AuNP. Procedure Creating the Gold Stock Solution Add 0.010048 g of HAuCl4·3H2O to a 10 mL volumetric flask and fill with distilled water. The final concentration of gold stock was 2.53 mM. Creating the Lysozyme Stock Solution Add 0.00570 g of lysozyme to a 20 mL volumetric flask and fill with distilled water. The final concentration of lysozyme stock was 0.02 mM. Synthesizing Lysozyme-AuNP Solutions with the following Au:protein ratio were synthesized: Gold to Lysozyme Ratio 40 50 110 Figure 1 shows the exact volumes of each stock solution added to a test tube. The test tubes were capped with aluminum foil before heated. The samples were heated at 80°C for 4 hours. Figures