Matthew E. Jurek Week 11: Difference between revisions
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#*Microarray data was analyzed using Fisher's exact test with a Bonferroni correction in order to acheive a p value threshold of .01. | #*Microarray data was analyzed using Fisher's exact test with a Bonferroni correction in order to acheive a p value threshold of .01. | ||
#*This data was then compared to previously published low-temperature transcriptome datasets. | #*This data was then compared to previously published low-temperature transcriptome datasets. | ||
#Results | |||
#*Biomass and fermentation rates did not differ significantly between cultures grown at 12 and 30 degrees C in carbon and nitrogen limited environments. | |||
#*Glucose-limited cultures: 494 genes yielded a significant difference amongst transcript levels at the two temps | |||
#*Nitrogen-limited cultures: 806 genes exhibited this type of behavior | |||
#*235 genes showed a change in regulation when both were limited | |||
#*Hextose transporter genes HXT2, HXT3, and HXT4 exhibited increased transcript levels at 12 degrees C | |||
#*HXT5 and HXT16 showed reduced transcription at this temp | |||
#*There are 3 genes that encode ammonia permeases. In the ammonium-limited cultures at 12 degrees C, 2 were reduced and one showed increased transcription. | |||
#*Important to note the specific growth rate of .03/h in this experiment is close to the maximum specific growth rate at 12 degrees versus 30 degrees | |||
#*Trehalose accumulation is a sign of low-temp adaptation | |||
#*However, there was not an increase in transcription of genes that metabolize trehalose and glycogen in this experiment | |||
#*Thus, the accumulation of trehalose and glycogen is not necessary for acclimation at low temps in yeast | |||
#*To date, the exact relevance of storage carbohydrate metabolism during adaptation remains a mystery. | |||
#*There was a noted up-regulation of translational machinery at low temps, especially 12 degrees C. | |||
#*Increased protein content could be a compensation mechanism for reduced enzyme kinetics at low temps. | |||
#*Three pre-existing datasets show 259 genes that responded to temp downshifts. | |||
#*Genes within these datasets were observed for up-regulation or down-regulation in the given conditions. | |||
#*Three genes consistently up-regulated at low temps were involved in lipid metabolism while three genes consistently down-regulated were involved in encoding transporters. | |||
#*Multiple stimuli are often responsible for changes in gene regulation. | |||
#*Batch culture results are unreliable as specific growth rate fluctuates with temperature and oxygen solubility is impacted by temperature. | |||
#*Genes that appear to signal cold shock in batch cultures may exhibit different behaviors in chemostat cultures. | |||
#*An ESR mechanism has been proposed in the regulation of yeast genes that exhibit transcriptional changes in response to multiple environmental stresses. | |||
#*In this study, it was found that ESR is NOT a response to growth at low temperature. ESR occurs following sudden exposure to suboptimal temps. |
Revision as of 22:09, 3 April 2013
Matthew E. Jurek BIOL398-03/S13
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- Final Presentation
Biological Terms
- trehalose- also known as mycose or tremalose, is a natural alpha-linked disaccharide formed by an α,α-1,1-glucoside bond between two α-glucose units.
- mannoprotein- component of yeast cell walls; protein covalently linked to polymers of mannose.
- chromatography- the separation of mixtures into their constituents by preferential adsorption by a solid, as a column of silica (column chromatography) or a strip of filter paper (paper chromatography) or by a gel.
- immunoprecipitation- the separation of an antigen from a solution by the formation of a large complex with its specific antibody.
- catabolite- a product of catabolic action.
- kinetics- the branch of mechanics that deals with the actions of forces in producing or changing the motion of masses.
- ceramides- ceramides are a family of lipid molecules. A ceramide is composed of sphingosine and a fatty acid.
- orthologue- one of two or more homologous gene sequences found in different species.
- ergosterol- a compound present in ergot and many other fungi. A steroid alcohol, it is converted to vitamin D2 when irradiated with ultraviolet light.
- transcriptome- the transcriptome is the set of all RNA molecules, including mRNA, rRNA, tRNA, and non-coding RNA produced in one or a population of cells.
Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analyis
Tai et al. (2007) Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis. Molecular Biology of the Cell 18: 5100–5112.
- Introduction
- On the cellular level, yeast responds in a number of ways to temperature changes (temps outside the 25-30 degree C optimum).
- Temperatures below the optimum range slow cellular processes.
- Effects of low temperature depend on exposure time
- Sudden exposure results in adaptaion
- Prolonged exposure results in acclimation
- Previous studies have focused on cold shock (sudden exposure) and found 2 phases of cold-shock response
- Current low-temp transcriptome databases contain major discrepancies
- Previous studies have focused on batch culutures: hard to distinguish temp effects on transcription from effects of specific growth rate
- Chemostat cultures allow for control of specific growth rate independent of culture conditions such as metabolites, pH, and oxygen availability.
- Overall Goal: Focus on genome-wide transcriptional regulation by exploring steady-state acclimatized growth of yeast at low temps
- Materials and Methods
- Haploid strain CEN.PK113-7D was grown at both 12 and 30 degrees C, anaerobically, in a chemostat
- The chemstat contained a dilution rate of .03/h, pH of 5.0 and stirrer speed of 600rpm.
- The cultures were contained in a synthetic medium that limited either carbon or nitrogen. Every other requirement was in excess.
- Each growth condition consisted of 3 independent replicates.
- Microarray data was analyzed using Fisher's exact test with a Bonferroni correction in order to acheive a p value threshold of .01.
- This data was then compared to previously published low-temperature transcriptome datasets.
- Results
- Biomass and fermentation rates did not differ significantly between cultures grown at 12 and 30 degrees C in carbon and nitrogen limited environments.
- Glucose-limited cultures: 494 genes yielded a significant difference amongst transcript levels at the two temps
- Nitrogen-limited cultures: 806 genes exhibited this type of behavior
- 235 genes showed a change in regulation when both were limited
- Hextose transporter genes HXT2, HXT3, and HXT4 exhibited increased transcript levels at 12 degrees C
- HXT5 and HXT16 showed reduced transcription at this temp
- There are 3 genes that encode ammonia permeases. In the ammonium-limited cultures at 12 degrees C, 2 were reduced and one showed increased transcription.
- Important to note the specific growth rate of .03/h in this experiment is close to the maximum specific growth rate at 12 degrees versus 30 degrees
- Trehalose accumulation is a sign of low-temp adaptation
- However, there was not an increase in transcription of genes that metabolize trehalose and glycogen in this experiment
- Thus, the accumulation of trehalose and glycogen is not necessary for acclimation at low temps in yeast
- To date, the exact relevance of storage carbohydrate metabolism during adaptation remains a mystery.
- There was a noted up-regulation of translational machinery at low temps, especially 12 degrees C.
- Increased protein content could be a compensation mechanism for reduced enzyme kinetics at low temps.
- Three pre-existing datasets show 259 genes that responded to temp downshifts.
- Genes within these datasets were observed for up-regulation or down-regulation in the given conditions.
- Three genes consistently up-regulated at low temps were involved in lipid metabolism while three genes consistently down-regulated were involved in encoding transporters.
- Multiple stimuli are often responsible for changes in gene regulation.
- Batch culture results are unreliable as specific growth rate fluctuates with temperature and oxygen solubility is impacted by temperature.
- Genes that appear to signal cold shock in batch cultures may exhibit different behaviors in chemostat cultures.
- An ESR mechanism has been proposed in the regulation of yeast genes that exhibit transcriptional changes in response to multiple environmental stresses.
- In this study, it was found that ESR is NOT a response to growth at low temperature. ESR occurs following sudden exposure to suboptimal temps.