Difference between revisions of "Ketner Lab"

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== Current Projects ==
 
== Current Projects ==
 
'''Adenovirus Vaccines'''
 
'''Adenovirus Vaccines'''
*Malaria
+
*[[Malaria]]
 
*HPV
 
*HPV
  
 
'''Ubiquination'''
 
'''Ubiquination'''
  
 
----
 
  
 
== Protocols ==
 
== Protocols ==
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*Calculate MOI
 
*Calculate MOI
 
*Protein preps of infected 293 cells for westerns
 
*Protein preps of infected 293 cells for westerns
*Western Blots
+
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
 
*P32 label DNA
 
*P32 label DNA
 +
*Quantitate number of virions in CsCl virus preps
 +
 +
==Materials==
 +
===Antibodies===
 +
*[[anti-HA]]
 +
*[[anti-Fiber]]
 +
*[[anti-Late serum]]
 +
*[[anti-NANP hybridoma supernatant]]
 +
 +
===Plasmids===
 +
===Viruses===
 +
===Cells===
 +
 +
== Links ==
 +
[http://faculty.jhsph.edu/?F=Gary&L=Ketner Gary Ketner]
 +
 +
[http://www.jhsph.edu/PublicHealthNews/Magazine/ JHSPH Magazine]

Latest revision as of 12:55, 20 April 2008

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA
  • Quantitate number of virions in CsCl virus preps

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine