Ketner Lab

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Current Projects

Adenovirus Vaccines

• Malaria
• HPV

Ubiquination

Protocols

• Adenovirus CaCl transfection
• Generating hexon mutants in E. coli
• Generating fiber mutants in E. coli
• Generating late gene expression mutants in 293 cells
• Purifying adenovius (banded stocks)
• Titration of viral stocks (calculate pfu)
• Calculate MOI
• Protein preps of infected 293 cells for westerns
• Western Blot
• P32 label DNA
• Quantitate number of virions in CsCl virus preps

Materials

Antibodies

• anti-HA
• anti-Fiber
• anti-Late serum
• anti-NANP hybridoma supernatant

Links

Gary Ketner

JHSPH Magazine