IGEM:Peking/2007: Difference between revisions
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'''Protocols''' | '''Protocols''' | ||
[[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br> | [[iGEM:Peking/2007/Oligos | Oligonucleotides]]<br> | ||
[[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Agarose Gel Electrophoresis]]<br> | [[iGEM:Peking/2007/Count:Agarose Gel Electrophoresis| Agarose Gel Electrophoresis]]<br> | ||
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[[iGEM:Peking/2007/Transformation | Transformation]]<br> | [[iGEM:Peking/2007/Transformation | Transformation]]<br> | ||
[[iGEM:Peking/2007/Sequencing | Sequencing]]<br> | [[iGEM:Peking/2007/Sequencing | Sequencing]]<br> | ||
[[iGEM:Peking/2007/Switch:Miller | Switch: Miller Method]]<br> | [[iGEM:Peking/2007/Switch:Miller | Switch: Miller Method]]<br> | ||
[[iGEM:Peking/2007/Count:Miniprep | Count: Miniprep]]<br> | [[iGEM:Peking/2007/Count:Miniprep | Count: Miniprep]]<br> | ||
[[iGEM:Peking/2007/Count:Conjugation| Count: Conjugation test]]<br> | [[iGEM:Peking/2007/Count:Conjugation| Count: Conjugation test]]<br> | ||
[[iGEM:Peking/2007/Count:Knockout| Count: Gene Knock Out]]<br> | |||
Revision as of 20:47, 20 August 2007
There are<html> <script language="JAVASCRIPT"><!-- update your browser, silly--> Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script> </html>days left until the Jamboree! Hop Count with Conjugation
Hop counting is a cell-cell communication system in which the signals transfered among the cells record the number of times of transfer. We decided to use conjugation as our one to one cell-cell communication system and plasmids as the vector for the transfered signal.
With the rolling-cycle replication of conjugation, independent tandem oriT (<bbpart>BBa_J01002</bbpart> and more) pairs can be inserted into the same plasmids, losing one tandem region at each single conjugation event.
Sequential conjugation and deletion of the different oriT pairs can be achieved by fine control of the expression of the cis-acting elements of the independent conjugation systems.
Besides intellectual interests, these systems are the core parts of controllable and encoded local cell-cell communication, which can be used in potential pattern formation and material weaving applications. |
<html> <img src="http://openwetware.org/images/b/b9/Icon_board.png" alt="Project"> </html>ProjectHop Count with Conjugation Push_on_push_off Switch Meetings
Brainstorming
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<html> <img src="http://openwetware.org/images/e/e2/Icon_info.png" alt="News" border="0"> </html>LabProducts and Parts Procedures Protocols
Oligonucleotides Switch: Miller Method
Notebooks
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<html> <img src="http://openwetware.org/images/3/39/Icon_groups.png" alt="People"> </html>PeopleThe iGEM 2007 PKU Team now consists of about 20 students and several faculty members from School of Life Science and Center of Theoretical Biology. StudentsPlease sign up here. Advisors
Photo |
Email us at igem-pku AT googlegroups DOT com
External links iGEM | iGEM 2006 | Registry of Standard Biological Parts | iGEM PKU Googlegroup | iGEM OWW Page