IGEM:IMPERIAL/2006/project/Oscillator/project browser/Prey Construct/Implementation: Difference between revisions
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==Ligation Strategy== | ==Ligation Strategy== | ||
<u><font size="2">'''Ligating the Prey Construct:'''</font></u> | |||
__NOTOC__ | |||
{| border="1" cellpadding="0" width="100%" | |||
* | |- | ||
!style="background:#E6B3E0" height="25pt" width="80pt" |For !! style="background:#E6B3E0" |Stage 1 !! style="background:#E6B3E0" |Stage 2 | |||
|- | |||
!style="background:#D9F2ED"|<center><bbpart>J37034</bbpart>=<br>(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>)</center> !!style="background:#CCEBAD" | <center>[[Image:C0261 logo.png]][[Image:Plus sign.png|20px]][[Image:I13504 logo.png]]</center> | |||
|- | |||
!style="background:#D9F2ED"|<center><bbpart>J37015</bbpart>=<br>(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>)</center>|| !!style="background:#CCEBAD" | <center>[[Image:J37015 logo.png]]</center> | |||
|} | |||
==Quality control== | |||
__NOTOC__ | |||
{| border="1" cellpadding="0" width="100%" | |||
|- | |||
!height="25pt" width="80pt"| Part !! Logo !! Built !! Sequenced !! Primers Used for Sequencing | |||
|- | |||
| <center><bbpart>J37034</bbpart></center> || [[Image:J37034 logo.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> | |||
|- | |||
| <center><bbpart>J37015</bbpart></center> || [[Image:J37015 part.png]] || Yes || <font color=red>'''?'''</font> || <font color=red>'''?'''</font> | |||
|} | |||
==WetLab work entries== | |||
*Part has been successfully built. | |||
*Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested. |
Revision as of 19:21, 29 October 2006
Super Parts | Molecular Prey-Predator Oscillator | ||
---|---|---|---|
Actual Part | |||
Sub Parts | Test Sensing Prey Construct | <bbpart>C0261</bbpart> | <bbpart>I13401</bbpart> |
Ligation Strategy
Ligating the Prey Construct:
For | Stage 1 | Stage 2 |
---|---|---|
(<bbpart>C0261</bbpart>+<bbpart>I13504</bbpart>) |
||
(<bbpart>F2620</bbpart>+<bbpart>J37034</bbpart>) |
Quality control
Part | Logo | Built | Sequenced | Primers Used for Sequencing |
---|---|---|---|---|
Yes | ? | ? | ||
Yes | ? | ? |
WetLab work entries
- Part has been successfully built.
- Pops blocker (for control of positive feedback loop) has been successfully made and needs to be incorporated into the prey part and tested.