Difference between revisions of "Erickson:Protocols"

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<br>
 
[[Erickson:TAE Buffer|TAE Buffer]]
 
[[Erickson:TAE Buffer|TAE Buffer]]
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[[Erickson:TB]]
 
==Antibiotics==
 
==Antibiotics==
 
[[Erickson:Kanamycin|Kanamycin]]
 
[[Erickson:Kanamycin|Kanamycin]]

Revision as of 16:23, 14 May 2009

PCR

Colony PCR
Real-Time PCR

RNA

RNA Isolation Protocol (TRIzol)

RNA Isolation Protocol (Mini Shredder)

Fleas

Flea Bedding

Media

LB mixture (includes plates mix)
LB Buffer
TAE Buffer
Erickson:TB

Antibiotics

Kanamycin
Carbenacillin
Tetracycline
Irgasan
Chloramphenicol

Transposon Mutagenesis

Tri-parental Mating

Electroporation

Bi-parental Mating

Mutant Generation

File:Making a mutant.pptx

Competent Cell Generation

Competent Cells