BISC220/S14: Mod 1 Media: Difference between revisions
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[[BISC220/S14: Mod 1 Lab 1 | Lab 1: Introduction to BISC220 Lab]]<br> | |||
[[BISC220/S14: Mod 1 Lab 2 | Lab 2: Induction of β-galactosidase and Molecular Modeling]]<br> | |||
[[BISC220/S14: Mod 1 Lab 3 | Lab 3: Affinity Chromatography and Polyacrylamide Gel Electrophoresis]]<br> | |||
[[BISC220/S14: Mod 1 Lab 4 | Lab 4: Specific Activity Determination]]<br> | |||
[[BISC220/S14: The Michaelis-Menten Model | The Michaelis-Menten Model]]<br> | |||
[[BISC220/S14: Mod 1 Lab 5 | Lab 5: Enzyme Kinetics]]<br> | |||
[[BISC220/S14: Mod 1 Media | Media Recipes]]<br> | |||
[[BISC220/S14: Mod 1 Science Writing | Science Writing and Figure Design]]<br> | |||
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== β-galactosidase Series Media Recipes == | == β-galactosidase Series Media Recipes == | ||
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Latest revision as of 07:43, 5 June 2013
β-galactosidase Series Media Recipes
Solution | Composition |
---|---|
Luria Broth | 1% Tryptone 0.5% Yeast Extract 1% NaCl 2% agar (only in plates) |
Ampicillin | Stock = 25 mg/ml in water |
IPTG (Isopropyl β-D-1-thiogalactopyranoside) | Stock 1 mg/ml |
Na2CO3 | Stock 1M |
Z-buffer | 60 mM Na2HPO4 60 mM NaH2PO4 1 mM MgSO4 10 mM HCl 0.27% β-mercaptoethanol |
ONPG (ortho-Nitrophenyl-β-galactoside) | Stock 4 mg/ml |
Laemmli Solution (2x Sample Buffer) | 0.125 M Tris, pH 6.7 4% SDS 20% glycerol 10% β-mercaptoethanol |
Gel Running Buffer | 0.92 M Glycine 0.05 M Tris Base 0.1% SDS |
GelCode Blue Stain Reagent | Thermo Scientific |