Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

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Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100 2. Spread 7.5 μL on each slide 3. Allow coverslips to dry 4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes


2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL