IGEM:Berkeley/2010

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Berkeley iGEM 2010

Gel Pictures

Project Name

Project Summary

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Undergrads
Amy Kristofferson http://openwetware.org/wiki/User:Amy_N._Kristofferson
Conor McClune
Tahoura
Daniela
Christoph

Graduate Students
Tim Hsiau

Postdocs

Insert names here

Advisors
Chris Anderson
Terry Johnson

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Lab calendar

The Details

Oligonucleotides
Sequences
Construction Files
-80 Stocks
Sequencing

Procedures

Polymerase Reactions
Wobble Reaction
Cloning by PCR
SOEing PCR
PCA Gene Synthesis
QuikChange

Cleanup Reactions
Regular Zymo Cleanup
Small-Frag Zymo Cleanup
Zymo Gel Purification

Digestions Reactions
EcoRI/BamHI Digest of Wobble Products
EcoRI/BamHI Digest of PCR Products
BglII Digest of EIPCR Products
Ligation of EcoRI/BamHI digests
Ligation of EIPCR digests
Analytical digests (Mapping)

Bacteria Manipulations
Transformation by heat-shock
Picking of colonies
Miniprep purification of DNA
Macherey-Nagel Miniprep

Other
LR Gateway Transfers
EcoRI/BamHI Part Transfers
What do I do with my sequencing data?
Small Scale Competent Cell Transformation
ELISA


Subgroup Notebooks



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Addressable Cell-to-Cell Communication

Useful Links


Subgroup Strategies, Overview

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