User:Allison K. Alix/Notebook/Thesis Research/2013/07/18: Difference between revisions
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29.67 x 10<sup>-9</sup>mol/L x 144.4x10<sup>-6</sup>L x 6.02x1023 AuNP/mol = 2.579x10<sup>12</sup> AuNP | 29.67 x 10<sup>-9</sup>mol/L x 144.4x10<sup>-6</sup>L x 6.02x1023 AuNP/mol = 2.579x10<sup>12</sup> AuNP | ||
'''Calculating the Total Surface Area Available for DNA Attachment''' | '''Calculating the Total Surface Area Available for DNA Attachment''' | ||
2.579x10<sup>12</sup>AuNP x 3.14x10<sup>-16</sup>m<sup>2</sup>/1 AuNP = 0.00081 m<sup>2</sup> | 2.579x10<sup>12</sup>AuNP x 3.14x10<sup>-16</sup>m<sup>2</sup>/1 AuNP = 0.00081 m<sup>2</sup> | ||
'''Calculating the Number of Moles of DNA Available to Attach''' | '''Calculating the Number of Moles of DNA Available to Attach''' | ||
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2.225x10<sup>-6</sup>moles/L x 144.4x10<sup>-6</sup>L = 3.2129x10<sup>-10</sup> moles | 2.225x10<sup>-6</sup>moles/L x 144.4x10<sup>-6</sup>L = 3.2129x10<sup>-10</sup> moles | ||
Calculating oligonucleotide density on each particle (theoretical) | |||
'''Calculating oligonucleotide density on each particle (theoretical)''' | |||
3.2129x10<sup>-10</sup> moles/0.00081 m<sup>2</sup> AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) | 3.2129x10<sup>-10</sup> moles/0.00081 m<sup>2</sup> AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP) | ||
'''Calculating oligonucleotide density on each particle (actual)''' | |||
moles DNA = (1.16872x10<sup>-7</sup>M x 300 x 10-6L) = 3.51x10<sup>-11</sup> moles | |||
3.51x10<sup>-11</sup> moles/0.00081 m<sup>2</sup> AuNP = 4.33x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP | |||
'''Calculating the percentage of DNA hybridized in each sample''' | |||
4.33x10<sup>-8</sup>moles DNA/m<sup>2</sup>AuNP/3.967x10-7 moles DNA/m2 AuNP = 10.9% | |||
moles DNA | |||
moles DNA | |||
Revision as of 08:17, 19 July 2013
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%DNA hybridized on AuNP from 7/16Calculating the surface area of 1 AuNP Surface Area of a sphere = 4πr2, r = 5nm 4π(5x10-9m)2 = 3.14 x 10-16m2 = 314nm2 Calculating the # of AuNP in 144.4uL 29.67nM solution 29.67 x 10-9mol/L x 144.4x10-6L x 6.02x1023 AuNP/mol = 2.579x1012 AuNP
2.579x1012AuNP x 3.14x10-16m2/1 AuNP = 0.00081 m2
2.225x10-6moles/L x 144.4x10-6L = 3.2129x10-10 moles
3.2129x10-10 moles/0.00081 m2 AuNP = 3.967x10-7 moles DNA/m2 AuNP (OR 39.67pmol DNA/cm2 AuNP)
moles DNA = (1.16872x10-7M x 300 x 10-6L) = 3.51x10-11 moles 3.51x10-11 moles/0.00081 m2 AuNP = 4.33x10-8moles DNA/m2AuNP
4.33x10-8moles DNA/m2AuNP/3.967x10-7 moles DNA/m2 AuNP = 10.9%
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