User:Allison K. Alix/Notebook/Thesis Research/2013/07/17

Absorbance Measurements of AuNP@SiO2

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To Do Take absorbance spectra of AuNP@SiO2 samples Determine which concentrations are suitable for TEM imaging (Thursday) Repeat DNA/AuNP extraction procedure using optimum centrifugation speed and time (10,000 rpm for 11 minutes) Obtain UV-Vis Data for DNA/AuNP samples and perform calculations following steps from User:Allison K. Alix/Notebook/Thesis Research/2013/07/16 DNA-AuNP attachment procedure Scale down to a final solution volume of 300uL due to minimal AuNP solution. Calculating volumes of buffer, AuNP, and DNA for final solution: [sodium citrate] = 268mM 268mM (x) = 10mM (300uL) x = 11.2uL sodium citrate (300uL-11.2uL)/2 = 144.4uL each of DNA and AuNP Procedures: 1) Prepare 200uL 4.45uM DNA 53.1uM(x) = 200uL(4.45) x = 16.8uL in 183.2uL 1X PBS 2) Mix DNA with 200uL DTT in TEA 3) React 10 minutes 4) Extract DNA using 4 2mL aliquots of ethyl acetate 5) Mix 144.4uL DNA with 144.4uL AuNP and 11.2uL sodium citrate 6) React 10 min 7) Centrifuge for 11 minutes @ 10,000rpm. Remove supernatant, redisperse in 50mM HEPES. Repeat 2 times.