20.20(S09):Teacher Resources

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Materials needed for teaching

2020(S09) Lecture:week 1

  1. Project Runway
    • paper, paperclips, tape, pennies, pencils, scissors
  2. iGEM reviews
    • copies of ppts from iGEM talks
  3. take apart
    • hard copies of Comic to cue homework assignment
    • tape recorders (16), tools (#0 phillips, scissors, wire cutters), bags to store parts, duct tape was suggested to collect small pieces, sharpies/paper/pens
    • link to pre-survey

2020(S09) Lecture:week 2

  1. tape recorder reassembly: bring electrical or duct tape next time to re-attach broken wires, bring 2xAA batteries, tape to play when players successfully reassembled.
  2. poster session studio: need 18"x24" paper, printer paper, felt tip markers, pencils, rulers, masking tape, sharpies
  3. play decide: print out games (genetic testing) onto proper colored sheets but do not cut. Instead, distribute sheets with scissors to allow students to cut and save just the cards they found most relevant. Depending on room, may also want to print out rules of play since screen not easy to see and tables too crowded for computers. Leave enough time so discussion can be recapped as well as policy.

2020(S09) Lecture:week 3

2020(S09) Lecture:week 4

  1. DNA isolation
    • Disposables: small white cups, larger clear plastic cups, coffee stirrerwater, Coffee filters, ziplock bags (sm for expt, lg for disposal), papertowels for spills
    • Reagents: strawberries, liquid soap, NaCl, isopropanol
  2. casting gel
    • tupperware: small rectangular (9.5 fl oz), larger rectangular for running (3 cup size)
    • Lego: for each need 1 (10 by 1) or(10 by 2) piece, 2 (2 by 3) piece, 6 (1 by 2) tiles
    • for each gel: 1/2 L bottle of water, salt, baking soda, aquarium pH paper, microwave, agar agar
    • misc: masking tape, scissors, coffee stirrer, paper coffee cup for melting in microwave
  3. loading gel
    • glycerin, food coloring, coffee stirrer
  4. running gel
    • for each gel: 5 9V batteries
  5. FooCamper's Guide
    • autoclaved beads - VWR 26396-521 for plating the cells.
    • hole punch to punch out the dNA
    • One-shot competent cells (I've gotten Invitrogen TOP10 previously, but NEB or whatever also works)
    • Ice in a bucket - for thawing cells
    • Glass of water, microwave and thermometer - for heat shock
    • SOC/LB media without antibiotic - for use after the heat shock - I aliquoted this out for them beforehand
    • Tape - so they can tape their tubes to a warm place to grow for 1 hr, or they can just hold them in their hands
    • Amp Plates - for people doing RFP and luciferase transformations
    • Liquid Amp media - For media, I mixed LB-Amp media pack - Invitrogen Q600-20 $169.00/20 pouches with bottled water and added isoamyl alcohol to 5mM - use this with the banana cultures
    • 14 mL culture tubes - VWR 60819-728

2020(S09) Lecture:week 5

2020(S09) Lecture:week 6

2020(S09) Lecture:week 7

2020(S09) Lecture:week 8

2020(S09) Lecture:week 9

2020(S09) Lecture:week 10

2020(S09) Lecture:week 11

2020(S09) Lecture:week 12

2020(S09) Lecture:week 13

2020(S09) Lecture:week 14


Not long ago when biology meant
looking and prodding and fixing what’s bent
there were armies of scientists working away
to list what they found
and to know life’s display

But not all was well as they tried to discover
how life could be programmed since some but not other
experiments worked and each person designed
their template for learning
as they were inclined

“Share!” said the engineers “and try to remember
that others will use your work only whenever
the tools you develop are standard and simple
think of most screwthreads and
think of the wheel.

Maybe in this way life by design
could work out of the box and others will find
lots of interesting ways to build up from the bottom
making useful new parts
then new systems. No problem!”

By combining devices in new and fun ways
the biologists builders could spend their workdays
learning what’s out there and making new widgets
to responsibly meet
the needs of our planet.