User:Mike Barnkob

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Information

Protocols

Cloning

Bacterial work

Immunology

Tissue culture work

  • Media
  • Thawing cells
  • Freezing down cells
  • Split cells
  • Count cells
  • Harvest splenocytes
  • Harvest BMDCs
  • In vitro expansion of T cells
  • Soft agar assay

Visualization

  • Long-time co-culture and visualization with live-dead marker

Projects

I work on:

  • T cell and cancer cell interactions
  • Genetic manipulation of T cells

Past:

Liquid handling robot. An attempt to create a small liquid handling robot to do simple experiments with.

Bacto Bandage. 2009 SDU Denmark iGEM team. Our goal was to create an E. coli strain, which inhibits S. aureus biofilm formation in wounds by producing RNA III-inhibiting-peptide (RIP).

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