User:Mike Barnkob

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Information

  • Mike Barnkob
  • Weatherall Institute for Molecular Medicine, University of Oxford
  • Website: www.mikebarnkob.dk.

Protocols

Coloning

  • CRISPR / Cas9
    • Designing sgRNA oligos
    • Cloning oligos into backbone
    • Transforming bacteria with construct
    • Screening for inserts
  • Lentiviral work
    • Lentiviral production
    • Infecting target cells

Bacterial work

  • Making LB agar plates

Immunology

  • Flow cytometry
  • CFSE staining

Tissue culture work

  • Medium
    • R10
    • D10
    • DMEM for 293TLA cells
  • Thawing cells
  • Freezing down cells
  • Split cells
  • Count cells
  • Harvest splenocytes
  • Harvest BMDCs
  • In vitro expansion of T cells
  • Soft agar assay

Mouse work

  • Bleeding
  • Harvesting bone-marrow DC's

Visualization

  • Long-time co-culture and visualization with live-dead marker

Projects

I work on:

  • T cell and cancer cell interactions
  • Genetic manipulation of T cells

Past:

[[Mike_Barnkob:Projects/

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