User contributions for Mike Barnkob

9 November 2015

• 09:1309:13, 9 November 2015 diff hist +78‎ Mike Barnkob:Protocols/Immunology/Making tetramer ‎ →‎References current

18 August 2015

• 02:2002:20, 18 August 2015 diff hist −17‎ Mike Barnkob:Protocols/Formulars ‎No edit summary current

27 July 2015

• 00:1600:16, 27 July 2015 diff hist +211‎ Mike Barnkob:Protocols/Immunology/Enrichment from tumours ‎No edit summary current

20 July 2015

• 08:4008:40, 20 July 2015 diff hist +98‎ Mike Barnkob:Protocols/Immunology/Making tetramer ‎ →‎Protocol
• 08:3108:31, 20 July 2015 diff hist +3‎ Mike Barnkob:Protocols/Immunology/Making tetramer ‎No edit summary
• 08:3008:30, 20 July 2015 diff hist +839‎ N Mike Barnkob:Protocols/Immunology/Making tetramer ‎ New page: ← Front page ==Making Tetramers== Protocol for conjugating monomers to streptavidin-APC. ===Reagents and equipment=== # Monomers # Straptavidin-APC (or other st...
• 08:0608:06, 20 July 2015 diff hist +73‎ User:Mike Barnkob ‎No edit summary current

14 July 2015

• 03:2603:26, 14 July 2015 diff hist −11‎ Mike Barnkob:Protocols/Bacterial/LB agar plates ‎No edit summary current
• 03:1603:16, 14 July 2015 diff hist +15‎ Mike Barnkob:Protocols/Bacterial/LB agar plates ‎No edit summary

3 June 2015

• 07:4907:49, 3 June 2015 diff hist +172‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary current
• 07:3907:39, 3 June 2015 diff hist +70‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎ →‎Blocking, permeabilization and staining

1 June 2015

• 04:3104:31, 1 June 2015 diff hist +399‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary

31 May 2015

• 08:1308:13, 31 May 2015 diff hist +125‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary
• 08:1108:11, 31 May 2015 diff hist +246‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary

27 May 2015

• 03:4803:48, 27 May 2015 diff hist +611‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary
• 03:3903:39, 27 May 2015 diff hist +1,476‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary
• 03:0803:08, 27 May 2015 diff hist +949‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary
• 02:2902:29, 27 May 2015 diff hist −43‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎ →‎Reagents
• 02:2802:28, 27 May 2015 diff hist +3‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎ →‎Reagents
• 02:2702:27, 27 May 2015 diff hist +80‎ Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎No edit summary
• 02:2402:24, 27 May 2015 diff hist +1,671‎ N Mike Barnkob:Protocols/Visualization/Immunofluorescence on cell lines ‎ New page: ← Front page ==Immunofluorescence protocol== This is a protocol for doing IF (immunofluorescence) on adherent and non-adherent cell lines. This protocol was last...
• 01:3601:36, 27 May 2015 diff hist −16‎ User:Mike Barnkob ‎No edit summary

20 May 2015

• 05:0305:03, 20 May 2015 diff hist +457‎ N Mike Barnkob:Protocols/Visualization/Immunofluorescence on non-adherent cells ‎ New page: ← Front page ==Immunofluorescence on non-adherent cells== Protocol for fixing, staining and visualizing non-adherent cell lines. ===Reagents=== '''Fixation''': ... current
• 02:0202:02, 20 May 2015 diff hist +125‎ User:Mike Barnkob ‎No edit summary

18 May 2015

• 12:5912:59, 18 May 2015 diff hist −6‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:4807:48, 18 May 2015 diff hist +6‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:1107:11, 18 May 2015 diff hist +12‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:1007:10, 18 May 2015 diff hist −1‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:0907:09, 18 May 2015 diff hist +7‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:0907:09, 18 May 2015 diff hist +13‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:0807:08, 18 May 2015 diff hist +2‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 07:0707:07, 18 May 2015 diff hist +250‎ Mike Barnkob:Protocols/Formulars ‎No edit summary
• 06:4406:44, 18 May 2015 diff hist +396‎ N Mike Barnkob:Protocols/Formulars ‎ New page: ==Formulars== ← Front page Basic formulars for lab use. ===Conversions=== ===Converting mg/ml to mM=== Divide the concentration in mg/ml by the molecular weigh...
• 06:3506:35, 18 May 2015 diff hist +57‎ User:Mike Barnkob ‎No edit summary

30 April 2015

• 14:1114:11, 30 April 2015 diff hist −1,903‎ Mike Barnkob:Protocols/Immunology/Enrichment from tumours ‎No edit summary
• 13:5613:56, 30 April 2015 diff hist +59‎ Mike Barnkob:Protocols/Immunology/Flow/Generic protocol ‎No edit summary current
• 13:5413:54, 30 April 2015 diff hist +1,758‎ N Mike Barnkob:Protocols/Immunology/Flow/Generic protocol ‎ New page: ← Front page ==Generic protocol for flow== Basic protocol for doing flow. ===Reagents=== * Antibodies * CD16/CD32 antibody for Fc-block. * Live-dead stain. * [[...
• 13:3513:35, 30 April 2015 diff hist +63‎ Mike Barnkob:Protocols/Mediums ‎No edit summary current
• 13:3213:32, 30 April 2015 diff hist +1,822‎ N Mike Barnkob:Protocols/Immunology/Flow/General notes ‎ New page: ← Front page ==General notes on flow cytometry== ===Antibody selection=== * Selection bright fluorochrome (PE, brilliant violet, 488) for markers that are less e... current
• 13:2713:27, 30 April 2015 diff hist +232‎ User:Mike Barnkob ‎No edit summary

1 April 2015

• 01:5401:54, 1 April 2015 diff hist +30‎ Mike Barnkob:Protocols/Cloning/PCR ‎ →‎Protocol current
• 01:4401:44, 1 April 2015 diff hist +1‎ Mike Barnkob:Protocols/Cloning/PCR ‎No edit summary
• 01:3101:31, 1 April 2015 diff hist −4‎ Mike Barnkob:Protocols/Cloning/PCR ‎No edit summary

6 March 2015

• 10:5710:57, 6 March 2015 diff hist +125‎ Mike Barnkob:Protocols/Cloning/cDNA library from mRNA ‎No edit summary current
• 10:5510:55, 6 March 2015 diff hist +1,902‎ Mike Barnkob:Protocols/Cloning/cDNA library from mRNA ‎No edit summary
• 10:3110:31, 6 March 2015 diff hist −1‎ Mike Barnkob:Protocols/Cloning/Isolating mRNA from spleen ‎No edit summary current
• 10:3010:30, 6 March 2015 diff hist +451‎ Mike Barnkob:Protocols/Cloning/Isolating mRNA from spleen ‎No edit summary
• 10:2910:29, 6 March 2015 diff hist +266‎ Mike Barnkob:Protocols/Cloning/cDNA library from mRNA ‎No edit summary
• 10:2210:22, 6 March 2015 diff hist −76‎ Mike Barnkob:Protocols/Cloning/Isolating mRNA from spleen ‎No edit summary
• 10:2110:21, 6 March 2015 diff hist +147‎ Mike Barnkob:Protocols/Cloning/Isolating mRNA from spleen ‎No edit summary