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Objective
Finish making malachite green solutions from yesterday
List protocol for today's lab
Lab Protocol
- Measure UV-Vis spectrum of different malachite green solutions
- Each group needs to make 2 new solutions (using the available stock solutions) and to measure their absorption spectrum
- As a group, we'll plot the data and determine extinction coefficients.
- Create notebook entries that include graphs.
Procedure for cleaning cuvette
- UV-Vis is a non-damaging technique. Replace your sample in the proper container using a pipette.
- Rinse cuvette with water
- Rinse cuvette with sodium dodecyl sulfate (SDS) solution
- Rinse cuvette with water
- Rinse cuvette with 10% HCl solution
- Rinse cuvette with water
Data
- Stock solutions
- 4.78 ppm (3.79083 g of 63.1 ppm Malachite green stock, water added to a total mass of 50.0521 g)
- 2.59 ppm (4.75639 g of 26.8 ppm Malachite green stock, water added to a total mass of 49.2598 g)
- 1.03 ppm (1.86080 g of 26.8 ppm Malachite green stock, water added to a total mass of 48.6527 g)
- 0.637 ppm (1.1903 g of 26.8 ppm Malachite green stock, water added to a total mass of 50.0786 g)
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
Student Pages
RG page1
[Alex Lutz 2018/08/31]
User:Raquel Lara/Notebook/481-2018/2018/08/31
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Biomaterials Design Lab
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