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Objective
Measure the UV-Vis spectra of different malachite green solutions in order to create a standard absorption curve.
Description
- Measure UV-Vis spectrum of different malachite solutions.
- Create 2 new solutions using available stock solutions.
- Plot the data and determine extinction coefficients.
Procedure for UV-Vis
- Since UV-Vis is a non-damaging technique, the sample can be returned after testing.
- First clean the cuvette.
- Rinse cuvette with water
- Rinse cuvette with sodium dodecyl sulfate (SDS) solution
- Rinse cuvette with water
- Rinse cuvette with 10% HCl solution
- Rinse cuvette with water
- Add the sample to the cuvette to fill about 3/4 full.
- Insert cuvette into UV-Vis.
- After UV-Vis runs, save twice (once as a .spec file and another time as a .txt data table).
- Remove cuvette from UV-Vis and return sample to container.
Data
- Stock solutions of Malachite green made by Dr. Hartings:
- 63.1 ppm
- 26.8 ppm
- 4.78 ppm (3.79083 g of 63.1 ppm Malachite green stock, water added to a total mass of 50.0521 g)
- 2.59 ppm (4.75639 g of 26.8 ppm Malachite green stock, water added to a total mass of 49.2598 g)
- 1.03 ppm (1.86080 g of 26.8 ppm Malachite green stock, water added to a total mass of 48.6527 g)
- 0.637 ppm (1.1903 g of 26.8 ppm Malachite green stock, water added to a total mass of 50.0786 g)
- Newly created stock solution of Malachite green
- .347 ppm (.275g of 63.1 ppm Malachite green stock, water added to a total mass of 50.078g)
- 9.97 ppm (7.91g of 61.3 ppm Malachite green stock, water added to a total mass of 50.08g)
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Notes
As can be seen with the best fit line, the data did not have as strong of a linear correlation that we would expect from the data suggesting that there were a substantial amount of errors while recording the data. Remeasuring will be required in order to get a better line of best fit.
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