User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28

Biomaterials Design Lab

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Objective Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples. Description Add experimental record here. Include what, how, and why... Data Stock solution for class AgNO3 28.13 mg AgNO3 to 10.0 mL in water 169.87 g/mol 16.6 mM Myoglobin Sample Prep 250 μM AuCl3, 12.5 μM myoglobin, pH 4 176 μL 4.27 mM AuCl3 234 μL 160 μM myoglobin 300 μL 1mM HCl 2290 μL water UV-Vis of sample before and after measurement Fluorescence data Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements Notes Anneliese Faustino Hannah Harvey Sara Mattson Patrick Hampson Sarah Burkhard