User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29

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Objective

Run fluorescence scans for

  1. BSA, AuNP, pH 6, 80C
  2. Lysozyme, pH 5, 80C

BSA AuNP Fluorescence

3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

UV-Vis of sample

Image:20160929 mrh BSA AuNP UVVis.png

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Image:20190929 mrh BSAAuNP IntegratedI.png

Image:20190929 mrh BSAAuNP EmissionMax.png

Image:20190929 mrh BSAAuNP scans1.png

Image:20190929 mrh BSAAuNP scans2.png

Image:20190929 mrh BSAAuNP scans3.png


Lysozyme Fluorescence

3 mL Sample

  1. 0.5 μM Lysozyme
    1. Stock Concentration 210 μM
    2. Volume Added: 7.1 μL
  2. pH 5
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 30 μL
  3. Volume Water Added: 2963 μL

UV-Vis of sample

Image:20160929 mrh Lysozyme pH5 UVVis.png

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Image:20160929 mrh LysozymepH5 IntegratedI.png

Image:20160929 mrh LysozymepH5 EmissionMax.png

Image:20160929 mrh LysozymepH5 SelectedScans.png

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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