User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/27

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Fluorescence Purpose Use fluorescence to examine the rate at which myoglobin unfolds at pH4 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 1.0mM HCl 3mL total Au 4.57mM (0.25mM)(3.0mL)=(XmL)(4.57mM) XmL= .176mL= 176uL Au Myoglobin 160uM (12.5uM)(3.0mL)=(XmL)(160uM) X=0.234mL= 234uL Myoglobin HCl 1.0mM (3.0mL)(1.0mM)=(1.0mM)(XmL) XmL=3.0mL= 300uL HCl Water Volume of water= 3000uL- 176uL-234uL-300uL= 2290uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Solutions for UV-VIS preparation Stock Concentrations: Au 4.57mM Myoglobin 160mM Fructose 40.9mM 0.25 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 293 31 388 3788 5 50 0 0 293 31 388 4238 6 5 0 0 293 31 388 4283 7 0 0 0 293 31 388 4288 8 0 5 0 293 31 388 4283 9 0 50 0 293 31 388 4238 10 0 500 0 293 31 388 3788 11 0 0 5 293 31 388 4283 12 0 0 50 293 31 388 4238 0.75 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 293 92 388 3727 5 50 0 0 293 92 388 4177 6 5 0 0 293 92 388 4222 7 0 0 0 293 92 388 4227 8 0 5 0 293 92 388 4222 9 0 50 0 293 92 388 4177 10 0 500 0 293 92 388 3727 11 0 0 5 293 92 388 4222 12 0 0 50 293 92 388 4177 1.25 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 293 159 388 3666 5 50 0 0 293 159 388 4116 6 5 0 0 293 159 388 4161 7 0 0 0 293 159 388 4166 8 0 5 0 293 159 388 4161 9 0 50 0 293 159 388 4116 10 0 500 0 293 159 388 3666 11 0 0 5 293 159 388 4161 12 0 0 50 293 159 388 4116 Results After the sample was run through fluorescence, no color change had occurred indicating there was a source of error in the data collected, possibly from there being no gold in the sample prepared. The graphs for the incorrect data were still made and are shown below. The correct sample was run on 09/29/2016 by Matt Hartings and the correct graphs were created. Graphs For Incorrect Data Integrated intensity for Myoglobin templated Gold Nanoparticles at pH4 Maximum intensity for Myoglobin templated Gold Nanoparticles at pH4 Maximum Wavelength for Myoglobin templated Gold Nanoparticles at pH4 The graphs for the correct data were created by Matt Hartings. Data was collected and interpreted on 09/28/2016 Notes pH4 was already done on 08/31/2016 but the prepared solution had incorrect concentrations of myoglobin and gold NPs