User:Jakob Suckale

• Gavage error estimation - created 02/10
• Bromodeoxyuridine (BrdU) - created 01/10
• TE antigen retrieval and immunostaining - created 11/09

• Glycerol - created 11/09
• Antigen retrieval - extended, reorganised 10/09
• Primer Tm estimation methods - 9/09
• Critical micelle concentration - 7/09
• Significance levels - created 7/09

• Making RNA probes with T7 transcription - created 6/09
• In vitro transcription with T7 RNA polymerase - restarted 6/09
• RNA extraction using self-made guanidinium-acid-phenol reagents - created 2/09
• Publication fees - created 1/09
• Cell and tissue lysis hub - created 6/08

• Generating antibodies - created 4/08
• RNA extraction using trizol/tri - 2/08
• Finding cDNA clones - created 2/08
• Choosing reference genes for qPCR normalisation - created 2/08
• QRT-PCR - complete rework, numerous additions 01-05/08

• Getting started in Dresden - created 11/07
• JC Ledin 2007 Gender gap in science - created 10/07
• Papers (bibliography software) - created review of software - 10/07
• LaTeX template for PhD thesis - improved template, description, instructions - 9/07
• Word vs. LaTeX - *created*, comparison in table, conclusion, links - 8/07 (>2000 hits)

• Hardcore PubMeding (advanced PubMed searching) - *created* help page, MeSH, My NCBI,.. - 6/07
• How to present your research well [3] - *created*, tips, problems, links - 4/07
• guide to Excel for statistics [4] - *created*, common stats functions, t-test, links - 3/07
• representing data numerically [5] - *created*, mean/median, sd/sem, examples - 3/07
• authorship [6] - *created*, Vancouver & discussion - 3/07
• Protein purification tags [7] - *created*, list of tags - 3/07
• LacZ staining of cells [8] - *created*, protocol, images - 2/07
• Q-PCR - *created*, most now moved to QRT-PCR - 2/07 (>1000 hits)
• PCR techniques [9] - overview page *created* - 2/07
• PCR [10] - steps of a PCR, primer design, PCR cycle in more readable list form - 12/06

• TE - recipe corrected 9/09
• Nanodrop - measurement error 7/09

• Shipment on dry ice - 7/09
• EGTA - created 6/09
• SSC - extended 6/09
• Antibodies - created 6/09
• Bromophenol blue - created 5/09

• Xylene cyanol - created 5/09
• SDS - created 4/09
• Tamoxifen - created 4/09
• MOPS - created 3/09
• Sterile filters - created 2/09

• RNA electrophoresis - additions 1/09
• Avoiding RNase contamination - additions 1/09
• DEPC - reworked 1/09
• Cresol Red - created 1/09
• Western blot (immunoblot) hub, Western Blot (immunoblot) - 10/08

• Triton - created 10/08
• Tween - created 10/08
• Antibodypedia - created 10/08
• Bioanalyzer - created 8/08
• Software for science reference management - created 7/08

• Making scientific posters - extended 7/08
• RNA blot (Northern) - created 3/08
• How to write a research paper - created 3/08
• RNA extraction - rework, added 2/08
• Scientific publishing - 1/08

• Skim - PDF reader and annotation software - 9/07
• Started with LaTeX on a Mac - 9/07
• LaTeX - rework 9/07
• Biosoftware for Mac - 9/07
• Powerpoint graphics for science presentations - 5/07

• tamoxifen administration to mice - 4/07
• EtOH precipitation of nucleic acids 4/07
• purification of DNA - 4/07
• BMJ Statistics Notes series - 4/07
• agarose gel loading dye - 4/07

• Hygromycin B - 4/07
• mouse tissue lysis for genotyping - 4/07
• polymethanal (PFA) - 4/07
• JC Lehmann 2006 Measuring scientific quality [11] - 4/07
• Journal Club [12] - 4/07

• R Statistics [13] - 3/07
• guide to statistics software - 3/07
• statistics portal - 3/07
• ethics portal - 3/07
• quantification of nucleic acids - 2/07

• gentamicin - 7/06
• aminoglycosides - 7/06
• G418 - 7/06
• neomycin - 7/06
• agarose gel electrophoresis - 6/06

Personal background

• currently working as postdoc in biology
• PhD in biology/experimental medicine at Max Planck Inst. Cell Biology and Genetics / Med Faculty, Dresden, Deutschland
• diploma thesis in bioinformatics - Università degli Studi di Padova, Italia
• biochemistry bachelor & master - Oxford University, UK

Drop me a line here on OpenWetWare

Member of the OWW Steering committee

Other wikis

• English wikipedia: my user page there

My OWW links

• Reviews
• OpenWetWare:Ideas
  • novel publishing
• OpenWetWare:Software
• OpenWetWare:Information management
• Special:OWWStats

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