User:Benjamin Friedel/Notebook/CHEM 471/2016/02/03

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Objective

Analyze results from 02/02/16 reactions (77 reactions), plan new reaction set for AgNP and AuNP formation with SPI, WPI, casein, pea protein, and brown rice protein. Record UVVis Spectra on successful NP reactions from 02/02/16.

Materials

  1. Used same nutritional proteins on 02/02/16
  2. Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable.
    1. Integration time= 10ms and Average =100.
    2. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples.
    3. Picture is shown on 02/02/16 page.

Procedure

Devised reaction set up to be prepared and ran on 02/02/16, not enough time today.

  1. Reducing stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions
  2. Hold protein volume and thus concentration) constant and change fructose concentration (reducing agent)

Detailed Reaction Setup for 02/02/16 Shown Below

1mL reactions ' ' ' ' ' ' ' '
2.5mM HAuCl4 (µL)2.5mMAgNO3 (µL)Protein (µL)2.5mM Fructose (µL)Water (µL)Au/Ag:ProteinNotes
.5mg/ml Casein140056004001:15
240044005201:11
340036006001:09
440028006801:07
540020007601:05
604056004001:15
704036006001:09
804020007601:07
1mg/ml SPI940056004001:15Formed white fibrous bundles 9-11 (precipitate?)
1040044005201:11
1140036006001:09
1240028006801:07purple nanofibers
1340020007601:05purple nanofibers
1404056006001:1514-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15040560205801:15
16040560405601:15
1704036006001:09
18040360205801:09
19040360405601:09
2004020006001:07
21040200205801:07
22040200405601:07
1mg/ml WPI2340056004001:15*All whey samples have double volume of protein
2440044005201:1123 (white precipitate at bottom)
2540036006001:09(24-27) white stringy fibers climbing tube walls
2640028006801:07
2740020007601:05
2804056006001:1528-30 clear
29040560205801:15
30040560405601:15
3104036006001:0931-33 clear
32040360205801:09
33040360405601:09
3404020006001:0734-36 clear
35040200205801:07
36040200405601:07
1mg/ml Pea Prot3740056004001:1537-40 purple and white fibers
3840044005201:11
3940036006001:09
4040028006801:07
4140020007601:0541 only purple fibers
4204056006001:1542-44 white precipitate at bottom
43040560205801:15
44040560405601:15
4504036006001:0945-47 white precipitate at bottom
46040360205801:09
47040360405601:09
4804020006001:0748-50 white preciptate at bottom
49040200205801:07
50040200405601:07
1mg/ml Brown Rice Prot5140056004001:1551-55 white precipitate with purple fibers near bottom of tube
5240044005201:11
5340036006001:09
5440028006801:07
5540020007601:05
5604056006001:1556-58 white preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
57040560205801:15
58040560405601:15
5904036006001:0959 - 60 white preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
60040360205801:09
61040360405601:0961 tan precipitate at bottom of tube, no fibers clinging to the sides
6204020006001:0759 - 60 white/yellow/black preciptate at bottom of tube and white particles (dots, fibers) clinging to walls
63040200205801:07
64040200405601:07



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