User:Benjamin Friedel/Notebook/CHEM 471/2016/02/02

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Objective

The objective for this experiment is to prepare gold and silver nanoparticles using nutritional soy protein isolate, micellar casein, whey protein isolate, pea protein isolate and brown rice protein isolate

Materials

Soy Protein Isolate (NutraBio) - http://www.nutrabio.com/category/spi/ Whey Protein Isolate (NutraBio) - http://www.nutrabio.com/product/WPI2/ Micellar Casein (NutraBio) - http://www.nutrabio.com/product/CAS2/ Pea Protein Isolate (Swanson Health Products) http://www.swansonvitamins.com/swanson-organic-certified-organic-pea-protein-powder-non-gmo-1-lb-454-grams-pwdr#label Sprouted Brown Rice Protein (Naked Nutrition) - http://nkdnutrition.com/products/organic-brown-rice-protein-powder

Protein concentrations are detailed on reaction setup. Concentrations were determined accurately through changing volume for a particular mass.

Procedure

This is the first day testing these nutritional proteins, the procedure from 01/27/16 is copied with these proteins as a starting point.

Reaction Setup Detailed below

' ' 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Au/Ag:Protein Notes
140056004001:15
240044005201:11
340036006001:09
440028006801:07
540020007601:05
604056004001:15
704036006001:09
804020007601:07
940056004001:15Formed white fibrous bundles 9-11 (precipitate?)
1040044005201:11
1140036006001:09
1240028006801:07purple nanofibers
1340020007601:05purple nanofibers
1404056006001:1514-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15040560205801:15
16040560405601:15
1704036006001:09
18040360205801:09
19040360405601:09
2004020006001:07
21040200205801:07
22040200405601:07
2340056004001:15*All whey samples have double volume of protein
2440044005201:1123 (white precipitate at bottom)
2540036006001:09(24-27) white stringy fibers climbing tube walls
2640028006801:07
2740020007601:05
2804056006001:1528-30 clear
29040560205801:15
30040560405601:15
3104036006001:0931-33 clear
32040360205801:09
33040360405601:09
3404020006001:0734-36 clear
35040200205801:07
36040200405601:07
3740056004001:1537-40 purple and white fibers
3840044005201:11
3940036006001:09
4040028006801:07
4140020007601:0541 only purple fibers
4204056006001:1542-44 white precipitate at bottom
43040560205801:15
44040560405601:15
4504036006001:0945-47 white precipitate at bottom
46040360205801:09
47040360405601:09
4804020006001:0748-50 white precipitate at bottom
49040200205801:07
50040200405601:07
5140056004001:1551-55 white precipitate with purple fibers near bottom of tube
5240044005201:11
5340036006001:09
5440028006801:07
5540020007601:05
5604056006001:1556-58 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
57040560205801:15
58040560405601:15
5904036006001:0959 - 60 white precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
60040360205801:09
61040360405601:0961 tan precipitate at bottom of tube, no fibers clinging to the sides
6204020006001:0759 - 60 white/yellow/black precipitate at bottom of tube and white particles (dots, fibers) clinging to walls
63040200205801:07
64040200405601:07
Whey 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Notes
23A400560040023A white fibers
24A400440052024A white fibers
25A400360060025A purple nanoparticles with dark pellet at bottom
26A400280068026A light purple (white and purple) nanofibers at bottom
27A400200076027A slightly pink solution nanoparticles
28A040560060028-33 clear
29A04056020580
30A04056040560
31A0403600600
32A04036020580
33A04036040560
34A040200060034A, cloudy white fibers at bottom of tube
35A04020020580clear
36A04020040560clear

Results

Graphs for particular gold and silver NP species by protein are shown below

  1. One new color type indicative of AuNP was made, purple Whey AuNP Solution, however, when UV vis spectra was taken, noting appeared. Its possible that protein did not dissolve fully and thus only very few NPs were made


Figure 1: UVVIS Spectra for CaseinAgNP Synthesized 02/02/15
Image:2.2.16 Casein AgNP.png

Note CaseinAg NP for this reaction time (4 hours 80˚C) peak at 420nm


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