User:Benjamin Friedel/Notebook/CHEM 471/2016/02/09

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Objective

  1. Setup AgNP and AuNP synthesis reactions for SPI, WPI, Casein, Pea Protein and Brown Rice Protein
  2. Further Research reducing sugars and other extra-protein reducing agents

Materials

  1. Used same nutritional proteins on 02/02/16
  2. Protein and gold stocks prepared fresh every day

Procedure

Reaction procedure devised on 02/03/16 prepared and executed today.

  1. Reducing stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions
  2. Hold protein volume and thus concentration) constant and change fructose concentration (reducing agent)

Detailed Reaction Setup for 02/09/16 Shown Below

1mL Reactions ' ' ' ' ' Note that many water volumes were too high, and therefore reactions were more dilute than antipated ' '
Tube #2.5mM HAuCl4 (µL)2.5mMAgNO3 (µL)Protein (µL)5mM Fructose (µL)Water (µL)Au/Ag:ProteinNotes
14005600400No change
2mg/mL Casein240056050350
grams weighed: 20mg3400560100300
Stock: 10mL40405600400No change
50403600600
60402000760
74005600400pink aggregate
8400560100300
94004400520darker pink/purple aggregate (maybe fibers)
0.75mg/mL SPI10400440100420
grams weighed: 7.5mg114002800680dark pink/purple fibers
Stock: 10mL12400280100580
130405600400white aggregate at bottom of tube
14040560100300
150404400520
16040440100420
170402800680
18040280100580
19400380062019-20 White aggregates at bottom of tube and sides
204003600640
21400340066021, slight pinkish tinge to white aggregates
1mg/mL Whey Protein220405600400No change
grams weighed: 10mg23040560100300
Stock: 10mL240403600600
25040360100500
260402000760
27040200100660
284003600640Dark purple specks at bottom of the tube (not much on walls)
2940036050590Lower protein concentration, as each lower volume of protein added, created darker and more particles
30400360100540
0.5mg/mL Pea Protein310405600400white aggregates at bottom of tube only
grams weighed: 10mg32040560100300
Stock: 10mL330404400520
34040440100420
350403600600
36040360100500
374003600640dark purple specks on bottom and sides of tube (mostly on bottom)
3840036050590- change protein to metal ratios, maybe change fructose to citrate
1mg/mL Rice Protein39400360100540
grams weighed: 10mg400405600400white aggregates at bottom of tube
Stock: 10mL41040560100300
420404400520
43040440100420
440403600600
45040360100500

Results

No reactions worked, this was due to some errors. Some water volumes were calculated higher than 1ml (all 1ml reactions). And thus everything in reaction mix was more dilute than necessary. Although the max this occurred at was 1.05ml/1.00ml.

No other reactions worked. Protein concentration was too high for casein (which was tested at 2mg/ml).


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