User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/11/24

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Contents

To do

  • Extractions of A. vaga
  • test extraction volumes

Extraction

  • 10, 20, 40 A. vaga in 200 ul GeneMatrix
  • standard heating scheme, with periodic agitation during extraction

PCR

Reagents 1x conc. 4
h2o5.75-23
10x110x4
dye110x4
dNTP0.210 mM0.8
Primers05 uM4
Taq0.055 U/um0.2
DNA25 ng/ul
TOTAL10Cocktail per vial9
  • 3m 95C (10s 95C, 1m 60C)x35
+ (40 ng)10 20 40
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