User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/01

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Contents

Test Redesigned Primers

PCR


Reagents Concentration 1X 5X
H205.7528.75
10X Buffer10X15
dye10X15
dNTP10mM0.21
Primers5uM1**
Taq5U/uM0.050.25
*DNA 20ng/ul15
TOTAL1045
Cocktail per well = 9.0ul
* DNA Adenita vaga** PrimersAV10126 F2+R2
Adenita ricciae AV10094 F2+R2
HIS F1+R1
HIS F1+R2
PCR 95C3 min
95C10 sec
60C2 min
35 cycles
' vaga ricciae vaga ricciae vaga ricciae vaga ricciae '
1Kb Ladderwell 1well 2well 3well 4well 5well 6well 7well 81Kb Ladder
AV10126AV10126AV10094AV10094HISF1+R1HISF1+R1HISF1+R2HISF1+R2
load 2.0ul ladder and 3.0ul sample on 1%gel
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