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- IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week8/Chemical and Light (section P108+45 colony PCR, P1+mtrB test digest 8/11)(3477) Lane 3: mtrB(BB)+P97 #63 cut XP (2100) Lane 4: mtrB(BB)+P97 #62 cut XP (2100) Lane 5: mtrB(BB)+P97 #64 cut XP (2100) Lane 6: mtrB(BB)+P97 #67 cut...18 KB (1,328 words) - 11:10, 18 August 2008
- replicated (origins) Promoters that work (inducible, etc) Request for gifts Can mtr genes be regulated (eg lacZ)? Knocking out EnvZ and/or OmpR (together a bit...3 KB (495 words) - 23:23, 21 June 2008
- replicated (origins) Promoters that work (inducible, etc) Request for gifts Can mtr genes be regulated (eg lacZ)? Knocking out EnvZ and/or OmpR (together a bit...879 bytes (128 words) - 07:13, 20 June 2008
- mtrB: 46, 2:30 P1/3: 45, 3:15 Cph: 2:30...41 bytes (12 words) - 06:44, 28 August 2008
- analogues Heatwole et al. Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli...3 KB (368 words) - 19:47, 19 August 2009
- with: mtrB TOPO ES (8/15): 0 colonies (on KAN) mtrB BB ES (8/14): 3 colonies (on KAN) P97 SP (dephos) with: mtrB BB XP (8/14): TMTC (on AMP) mtrB TOPO...4 KB (571 words) - 20:11, 22 September 2008
- wt Shewie, mtrA, mtrB, and mtrC/omcA in Orianna's growth media overnight cultures RESULTS: OD readings: wt: -0.001 mtrA: 0.001 mtrB: 0.003 mtrC/omcA: 0.002...2 KB (250 words) - 09:18, 15 July 2008
- IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week10/Chemical and Light (section PCR of mtrB with RBS + mtrB primers)need to be held off (ie. getting into the pSC101 vector, and adding on mtrB or mtrA) until the issue is resolved either with site directed mutagenesis or...5 KB (324 words) - 22:51, 30 August 2008
- IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week7/Chemical and Light (section 8/4: Cph/EnvZ, P1, P3, mtrB not BB)for 30 cycles. If the colonies contained mtrB, a band at ~2.1 kb should appear. No colonies appeared to contain mtrB. All gels with 1kb plus ladder Pl05 (CphEnvZ...34 KB (1,206 words) - 17:06, 19 August 2008
- IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week9/Chemical and Light (section 105, mtrB Ligations/transformations 08/17)transformed into TOP10 cells. In retrospect, it appears that all the ligations with mtrB are wrong, since the digest was actually wrong. The annealing temperature...6 KB (656 words) - 06:55, 21 August 2008
- in previous day's test) Ran with the same parameters as July 3, but with mtrA Shewie Results: Current production on the same order of magnitude as wt Shewie...5 KB (710 words) - 07:45, 10 July 2008
- 12 samples): 180μL PCR supermix, 4μL mtrB-ApaLI-F primer (20μM), 4μL mtrB-KpnI-R-new (20μM), pipet tip touch of mtrB BB from gel purification, 12μL water...41 KB (1,426 words) - 21:16, 27 July 2008
- IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week6/Chemical and Light (section Transformed mtrB constitutive system into S1 7/28)for liquid cultures and glycerol stocks. Made ~250 aliquots of wildtype and mtrB- Shewie-- 80 uL in each aliquot. We digested several samples of P17 in the...27 KB (1,434 words) - 16:52, 14 August 2008
- Buy computer? anaerobic Shewie? manganese oxide? meet with Diane Newman mtrA mtrB both OmpR knockout Homologous recombination (Pacific Northwest?) Cre-Lox...733 bytes (63 words) - 12:34, 23 June 2008
- Shwanella oneidensis をモデルにした。 lactate→代謝→電子+酸素→水 この電子を出力に。 mtrBが水への反応を促進する(mtrBがあると100%水への反応起きるが、ないと25%位に) mtrBを抑制する回路を作る。 1、chemical inducible system IPTGの有り無し...6 KB (1,364 words) - 07:17, 16 March 2009
- Shewanella Δ EnvZ (which should still have mtrB). Rx mix: 90μL PCR supermix, 2μL mtrB-ApaLI-F primer (20μM), 2μL mtrB-KpnI-R (20μM), swirl of colony Rx: 10min...38 KB (2,107 words) - 09:57, 21 July 2008
- ESXmnI The XmnI is to cut the backbone, which has a similar length to the mtrB constructs. With Fermentas enzymes, 25 min: 116 SP 117 SP 108 SP 51 EX 63...3 KB (504 words) - 05:59, 4 October 2008
- create a point mutation in the pstI site in mtrA. Used 5ug of primer. 7/8/08 Used gradient PCR to PCR mtrB. Cycled 30-40°C x 10 and then 52-62°C x 30....30 KB (1,929 words) - 06:29, 1 August 2008
- Lab meeting Cleaned chambers started mtrB and wt cultures troubleshoot LabVIEW...210 bytes (11 words) - 07:26, 23 July 2008
- for experiment finished building 3 MFC's Set up LabVIEW Experiment: wt, 2 mtrB chambers added lactate overnight Note: Concluded indirect bubbling produces...898 bytes (95 words) - 08:51, 20 August 2008