IGEM:Harvard/2008/Lab Notebooks/DailyBook/Week1
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/Chemical and Light/
/Widgetry/
Monday Meeting Notes
Goals for Week 1
Light team
- knockout protocol
- cloning part
Chemical team
- transform registry DNA into Shewie
- confirm Shewie growth via PCR
Widget team
- create basic MFC protocol
- speak with Pete and confirm design
- think about computer interfacing
- LabView/MatLab?
- Buy computer?
Team Goals
- anaerobic Shewie?
- manganese oxide?
- meet with Diane Newman
Things to ask for
Mutants from Orianna
- mtrA
- mtrB
- both
UT Austin
- OmpR knockout
Protocols
- Homologous recombination (Pacific Northwest?)
- Cre-Lox (Orianna)
- Colorimetric assay (Orianna)
- Shewie transformation (Diane Newman)