IGEM:Stanford/2009/Project Homeostasis/Immunosuppression Device
|Sequences & Primers|
The anti-immunosuppressive device consists of two components: a sensor and a responder. The sensor will detect local concentrations of a marker indicative of Treg-driven immunosuppression. When the levels of this input signal reach above a certain baseline threshold, the sensor will induce activity from the responder. The resulting output signal will modify the local cytokine milieu to support differentiation of pro-inflammatory Th17 cells and repress the immunosuppressive Treg lineage.
- Rather than looking at metabolic changes due to Th17 cells, maybe we should look at precursor changes that result in Th17 differentiation. Think of it as a feed forward mechanism. I am thinking in particular things that are affected by Aryl Hydrocarbon Receptor (AHR) and RAR-related orphan receptor gamma (RORy).
- Check out this paper on Th17 And Treg control. http://www.nature.com/nature/journal/v453/n7191/abs/nature06880.html
To what extent will 5-methyltryptophan inhibit growth of our machine? 5-methyltryptophan has been identified as an antimetabolite that induces false feedback inhibition of the trp biosynthetic pathway.
Other potential "input" signals: IL-10, TGF-beta, byproducts of HO-1 Other potential "output" signals: IL-17, IL-1-beta, CpG DNA, TLR stimuli
Treg markers/possible input signals
- Oliveira et al. Regulatory T cell maintenance of dominant tolerance: induction of tissue self-defense? Transpl Immunol. 2006 Dec;17(1):7-10. Epub 2006 Oct 12. Mentions the small molecule markers produced as a result of Treg-induction of IDO and HO-1.
- Heatwole et al. Cloning, nucleotide sequence, and characterization of mtr, the structural gene for a tryptophan-specific permease of Escherichia coli K-12. J Bacteriol. 1991 January; 173(1): 108–115.
- Kuhn et al. Mutant Strains of Escherichia coli K-12 Exhibiting Enhanced Sensitivity to 5-Methyltryptophan. J Bacteriol. 1972 October; 112(1): 93–101.