User contributions for Nishant M. Bhat

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Results for Nishant M. Bhat talk block log uploads logs

A user with 295 edits. Account created on 25 April 2007.

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08:0108:01, 18 October 2010 diff hist −22 m User:Nishant M. Bhat No edit summary current
07:5907:59, 18 October 2010 diff hist +71 m User:Nishant M. Bhat No edit summary

06:2306:23, 3 August 2010 diff hist +97 User:Nishant M. Bhat No edit summary

13:3613:36, 14 January 2010 diff hist −28 User:Nishant M. Bhat No edit summary

02:5202:52, 16 January 2009 diff hist +9 m User:Nishant M. Bhat No edit summary
02:4702:47, 16 January 2009 diff hist +121 User:Nishant M. Bhat No edit summary

14:3314:33, 16 August 2007 diff hist +17 Registry/Measurement kit/Glycerol box No edit summary
14:3214:32, 16 August 2007 diff hist 0 Registry/Measurement kit/Glycerol box No edit summary
14:3214:32, 16 August 2007 diff hist +1 Registry/Measurement kit/Glycerol box No edit summary
14:3114:31, 16 August 2007 diff hist +501 Registry/Measurement kit/Glycerol box No edit summary

06:5506:55, 15 August 2007 diff hist +48 User:Jessica Karen Wong/Design →I2055 current
06:5206:52, 15 August 2007 diff hist +31 N File:I2055-design.JPG Diagram of RBS tester with GFP. current
02:2302:23, 15 August 2007 diff hist +117 Registry/Measurement kit/Notebook/2007-8-14 No edit summary current

17:2417:24, 14 August 2007 diff hist +189 N Registry/Measurement kit/Notebook/2007-8-14 New page: *PCR cleaned NotI digests of 56 and 55 (EX, SX) *re-circularizing ligation. *Sent some sequencing requests: **E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,...
16:5316:53, 14 August 2007 diff hist +86 N Registry/Measurement kit/Notebook/2007-8-13 New page: *Sequencing: **F2620 - 2 samples **I2055 - EX B32 **I2055 - SX B32 **I2057 - EX J23116 current

20:2220:22, 10 August 2007 diff hist +46 Registry/Measurement kit/Notebook/2007-8-10 No edit summary current
20:2120:21, 10 August 2007 diff hist +38 Registry/Measurement kit/Notebook/2007-8-10 →F2620
20:2120:21, 10 August 2007 diff hist 0 N File:F2620-8-10-07.jpg No edit summary current
19:5519:55, 10 August 2007 diff hist +103 N Registry/Measurement kit/Notebook/2007-8-10 New page: ==F2620== *Colony PCR gel: **#1 (8-1) _L_ #2(8-1) **#3 (8-1) _L ==I2056== *Sent EX, SX for sequencing

20:5420:54, 9 August 2007 diff hist +242 N Registry/Measurement kit/Notebook/2007-8-8 New page: thumb|left|PCR Digest Gel **Loaded: lad sp I2057-EX, EX Digest, I2057-ES, ES digest, I2056-EX, EX digest, I2056-SX, SX digest, I2055-EX, I2055 EX digest, sp, I2055 SX... current
20:5120:51, 9 August 2007 diff hist +143 Registry/Measurement kit/Notebook/2007-8-9 No edit summary current
20:2320:23, 9 August 2007 diff hist +338 N Registry/Measurement kit/Notebook/2007-8-9 New page: *Reviewed sequencing results: **None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. **F2620 isn't right, should try re-ligating and transformibg in DB3.1 **55-SX-B0031 was ba...

19:1119:11, 4 August 2007 diff hist +213 N Registry/Measurement kit/Notebook/2007-8-4 New page: *Transformed post-DpnI E0240 (hopefully mutated) (labeled E or M primer and 1 or 2 µL template.) *Made chemically competent MG1655 cels. *Digest of prepped I2057 was left in incubator too... current

20:5120:51, 3 August 2007 diff hist +27 Registry/Measurement kit/Notebook/2007-8-3 No edit summary current
20:4120:41, 3 August 2007 diff hist +279 Registry/Measurement kit/Notebook/2007-8-3 No edit summary
18:2418:24, 3 August 2007 diff hist +102 Registry/Measurement kit/Notebook/2007-8-3 No edit summary
18:1618:16, 3 August 2007 diff hist +31 N Registry/Measurement kit/Notebook/2007-8-3 New page: *Trying mutagenic PCR of E0240.

22:4022:40, 2 August 2007 diff hist +52 Registry/Measurement kit/Notebook/2007-8-2 No edit summary current
17:5617:56, 2 August 2007 diff hist +118 Registry/Measurement kit/Notebook/2007-8-2 No edit summary
16:3916:39, 2 August 2007 diff hist +97 N Registry/Measurement kit/Notebook/2007-8-2 New page: *Cleaning digests from yesterday. *Mini-prepping I2056 E-S, E-X *Running analytical gel of F2620.

20:4920:49, 1 August 2007 diff hist −3 Registry/Measurement kit/Notebook/2007-8-1 No edit summary current
19:0019:00, 1 August 2007 diff hist +1 Registry/Measurement kit/Notebook/2007-8-1 No edit summary
18:5318:53, 1 August 2007 diff hist +206 Registry/Measurement kit/Notebook/2007-8-1 No edit summary
18:4918:49, 1 August 2007 diff hist +45 Registry/Measurement kit/Notebook/2007-8-1 No edit summary
18:4618:46, 1 August 2007 diff hist +29 Registry/Measurement kit/Notebook/2007-8-1 →I2055
18:4518:45, 1 August 2007 diff hist +26 N File:8-1.jpg ladder, I2055-EX, I2055-SX current
18:4418:44, 1 August 2007 diff hist +48 Registry/Measurement kit/Notebook/2007-8-1 No edit summary
15:3715:37, 1 August 2007 diff hist +129 N Registry/Measurement kit/Notebook/2007-8-1 New page: ==I2057== *Will start digest of promoters. ==I2056== *Purified digests (NotI, E/X, S/X) ==I2055== *Running gel of BioBrick PCR.

21:5721:57, 31 July 2007 diff hist +111 Registry/Measurement kit/Notebook/2007-7-31 No edit summary current
21:1921:19, 31 July 2007 diff hist +710 N Registry/Measurement kit/Notebook/2007-7-31 New page: ==E0240== *Noticed that during the scarring PCR, a point substitution was made. *Decided to try a site-directed mutagenesis on the biobricked product. *Ordered two pairs of primers: **...
21:1121:11, 31 July 2007 diff hist +55 MfeI No edit summary current
21:1021:10, 31 July 2007 diff hist +63 NsiI No edit summary current
21:0821:08, 31 July 2007 diff hist +69 NsiI No edit summary
21:0821:08, 31 July 2007 diff hist +69 MfeI No edit summary

19:4619:46, 30 July 2007 diff hist +47 Registry/Measurement kit/Notebook/2007-7-30 No edit summary current
19:3219:32, 30 July 2007 diff hist −23 Registry/Measurement kit/Notebook/2007-7-30 No edit summary
16:1016:10, 30 July 2007 diff hist +87 Registry/Measurement kit/Notebook/2007-7-30 No edit summary
16:0816:08, 30 July 2007 diff hist +56 Registry/Measurement kit/Notebook/2007-7-30 No edit summary
16:0616:06, 30 July 2007 diff hist +45 Registry/Measurement kit/Notebook/2007-7-30 No edit summary
15:5915:59, 30 July 2007 diff hist +26 Registry/Measurement kit/Notebook/2007-7-30 →Promoter Testers

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