User contributions for Nishant M. Bhat

18 October 2010

• 01:0101:01, 18 October 2010 diff hist −22‎ m User:Nishant M. Bhat ‎No edit summary current
• 00:5900:59, 18 October 2010 diff hist +71‎ m User:Nishant M. Bhat ‎No edit summary

2 August 2010

• 23:2323:23, 2 August 2010 diff hist +97‎ User:Nishant M. Bhat ‎No edit summary

14 January 2010

• 06:3606:36, 14 January 2010 diff hist −28‎ User:Nishant M. Bhat ‎No edit summary

15 January 2009

• 19:5219:52, 15 January 2009 diff hist +9‎ m User:Nishant M. Bhat ‎No edit summary
• 19:4719:47, 15 January 2009 diff hist +121‎ User:Nishant M. Bhat ‎No edit summary

16 August 2007

• 07:3307:33, 16 August 2007 diff hist +17‎ Registry/Measurement kit/Glycerol box ‎No edit summary
• 07:3207:32, 16 August 2007 diff hist 0‎ Registry/Measurement kit/Glycerol box ‎No edit summary
• 07:3207:32, 16 August 2007 diff hist +1‎ Registry/Measurement kit/Glycerol box ‎No edit summary
• 07:3107:31, 16 August 2007 diff hist +501‎ Registry/Measurement kit/Glycerol box ‎No edit summary

14 August 2007

• 23:5523:55, 14 August 2007 diff hist +48‎ User:Jessica Karen Wong/Design ‎ →‎I2055 current
• 23:5223:52, 14 August 2007 diff hist +31‎ N File:I2055-design.JPG ‎ Diagram of RBS tester with GFP. current
• 19:2319:23, 14 August 2007 diff hist +117‎ Registry/Measurement kit/Notebook/2007-8-14 ‎No edit summary current
• 10:2410:24, 14 August 2007 diff hist +189‎ N Registry/Measurement kit/Notebook/2007-8-14 ‎ New page: *PCR cleaned NotI digests of 56 and 55 (EX, SX) *re-circularizing ligation. *Sent some sequencing requests: **E0240-E2-3,4; E0240-M1-2,3,4; I56-SX-4,8; I56-EX-2,8; I55-EX-2,3; I55-SX-4,...
• 09:5309:53, 14 August 2007 diff hist +86‎ N Registry/Measurement kit/Notebook/2007-8-13 ‎ New page: *Sequencing: **F2620 - 2 samples **I2055 - EX B32 **I2055 - SX B32 **I2057 - EX J23116 current

10 August 2007

• 13:2213:22, 10 August 2007 diff hist +46‎ Registry/Measurement kit/Notebook/2007-8-10 ‎No edit summary current
• 13:2113:21, 10 August 2007 diff hist +38‎ Registry/Measurement kit/Notebook/2007-8-10 ‎ →‎F2620
• 13:2113:21, 10 August 2007 diff hist 0‎ N File:F2620-8-10-07.jpg ‎No edit summary current
• 12:5512:55, 10 August 2007 diff hist +103‎ N Registry/Measurement kit/Notebook/2007-8-10 ‎ New page: ==F2620== *Colony PCR gel: **#1 (8-1) _L_ #2(8-1) **#3 (8-1) _L ==I2056== *Sent EX, SX for sequencing

9 August 2007

• 13:5413:54, 9 August 2007 diff hist +242‎ N Registry/Measurement kit/Notebook/2007-8-8 ‎ New page: thumb|left|PCR Digest Gel **Loaded: lad sp I2057-EX, EX Digest, I2057-ES, ES digest, I2056-EX, EX digest, I2056-SX, SX digest, I2055-EX, I2055 EX digest, sp, I2055 SX... current
• 13:5113:51, 9 August 2007 diff hist +143‎ Registry/Measurement kit/Notebook/2007-8-9 ‎No edit summary current
• 13:2313:23, 9 August 2007 diff hist +338‎ N Registry/Measurement kit/Notebook/2007-8-9 ‎ New page: *Reviewed sequencing results: **None of E0240 worked except E0240-EX, E2. Not sure about ES, M2. **F2620 isn't right, should try re-ligating and transformibg in DB3.1 **55-SX-B0031 was ba...

4 August 2007

• 12:1112:11, 4 August 2007 diff hist +213‎ N Registry/Measurement kit/Notebook/2007-8-4 ‎ New page: *Transformed post-DpnI E0240 (hopefully mutated) (labeled E or M primer and 1 or 2 µL template.) *Made chemically competent MG1655 cels. *Digest of prepped I2057 was left in incubator too... current

3 August 2007

• 13:5113:51, 3 August 2007 diff hist +27‎ Registry/Measurement kit/Notebook/2007-8-3 ‎No edit summary current
• 13:4113:41, 3 August 2007 diff hist +279‎ Registry/Measurement kit/Notebook/2007-8-3 ‎No edit summary
• 11:2411:24, 3 August 2007 diff hist +102‎ Registry/Measurement kit/Notebook/2007-8-3 ‎No edit summary
• 11:1611:16, 3 August 2007 diff hist +31‎ N Registry/Measurement kit/Notebook/2007-8-3 ‎ New page: *Trying mutagenic PCR of E0240.

2 August 2007

• 15:4015:40, 2 August 2007 diff hist +52‎ Registry/Measurement kit/Notebook/2007-8-2 ‎No edit summary current
• 10:5610:56, 2 August 2007 diff hist +118‎ Registry/Measurement kit/Notebook/2007-8-2 ‎No edit summary
• 09:3909:39, 2 August 2007 diff hist +97‎ N Registry/Measurement kit/Notebook/2007-8-2 ‎ New page: *Cleaning digests from yesterday. *Mini-prepping I2056 E-S, E-X *Running analytical gel of F2620.

1 August 2007

• 13:4913:49, 1 August 2007 diff hist −3‎ Registry/Measurement kit/Notebook/2007-8-1 ‎No edit summary current
• 12:0012:00, 1 August 2007 diff hist +1‎ Registry/Measurement kit/Notebook/2007-8-1 ‎No edit summary
• 11:5311:53, 1 August 2007 diff hist +206‎ Registry/Measurement kit/Notebook/2007-8-1 ‎No edit summary
• 11:4911:49, 1 August 2007 diff hist +45‎ Registry/Measurement kit/Notebook/2007-8-1 ‎No edit summary
• 11:4611:46, 1 August 2007 diff hist +29‎ Registry/Measurement kit/Notebook/2007-8-1 ‎ →‎I2055
• 11:4511:45, 1 August 2007 diff hist +26‎ N File:8-1.jpg ‎ ladder, I2055-EX, I2055-SX current
• 11:4411:44, 1 August 2007 diff hist +48‎ Registry/Measurement kit/Notebook/2007-8-1 ‎No edit summary
• 08:3708:37, 1 August 2007 diff hist +129‎ N Registry/Measurement kit/Notebook/2007-8-1 ‎ New page: ==I2057== *Will start digest of promoters. ==I2056== *Purified digests (NotI, E/X, S/X) ==I2055== *Running gel of BioBrick PCR.

31 July 2007

• 14:5714:57, 31 July 2007 diff hist +111‎ Registry/Measurement kit/Notebook/2007-7-31 ‎No edit summary current
• 14:1914:19, 31 July 2007 diff hist +710‎ N Registry/Measurement kit/Notebook/2007-7-31 ‎ New page: ==E0240== *Noticed that during the scarring PCR, a point substitution was made. *Decided to try a site-directed mutagenesis on the biobricked product. *Ordered two pairs of primers: **...
• 14:1114:11, 31 July 2007 diff hist +55‎ MfeI ‎No edit summary current
• 14:1014:10, 31 July 2007 diff hist +63‎ NsiI ‎No edit summary current
• 14:0814:08, 31 July 2007 diff hist +69‎ NsiI ‎No edit summary
• 14:0814:08, 31 July 2007 diff hist +69‎ MfeI ‎No edit summary

30 July 2007

• 12:4612:46, 30 July 2007 diff hist +47‎ Registry/Measurement kit/Notebook/2007-7-30 ‎No edit summary current
• 12:3212:32, 30 July 2007 diff hist −23‎ Registry/Measurement kit/Notebook/2007-7-30 ‎No edit summary
• 09:1009:10, 30 July 2007 diff hist +87‎ Registry/Measurement kit/Notebook/2007-7-30 ‎No edit summary
• 09:0809:08, 30 July 2007 diff hist +56‎ Registry/Measurement kit/Notebook/2007-7-30 ‎No edit summary
• 09:0609:06, 30 July 2007 diff hist +45‎ Registry/Measurement kit/Notebook/2007-7-30 ‎No edit summary
• 08:5908:59, 30 July 2007 diff hist +26‎ Registry/Measurement kit/Notebook/2007-7-30 ‎ →‎Promoter Testers