- See about making competent DB3.1 cells
- Test the top10 stock with a + control (pUC18)
- See what we can do with BB'd E0240 & I2057
- Should pick some promoters to drop in.
- R0040 + a strong promoter + a weak promoter
- Using <bbpart>R0040</bbpart> and two of Anderson's promoters: <bbpart>J23116</bbpart> (weak) and <bbpart>J23100</bbpart> (strong)
- Using Joey's transformation of the promoters.
- Doing directional-dependent colony PCR (E0040_Reverse and either VF or VR)