- Started digest of promoters (E/S).
- Started another culture
- To do: ligate R0040 into the digests from the PCR and transform.
- Purified digests (NotI, E/X, S/X)
- Re-sealed and transformed.
- Will test B0030, B0031, B0032, B0034
- Running gel of BioBrick PCR.
- (ladder, I2055-EX, I2055-SX)
- Both look right size.
- Restriction digests: E/X, S/X, and NotI.
- Re-did scarring PCR of F2620.