User contributions for Insect Nutrition and Metabolism

1 April 2024

• 09:3609:36, 1 April 2024 diff hist 0‎ Analysing NGS ‎No edit summary current
• 09:3609:36, 1 April 2024 diff hist +3‎ Analysing NGS ‎No edit summary
• 09:3609:36, 1 April 2024 diff hist +197‎ Analysing NGS ‎No edit summary
• 09:3409:34, 1 April 2024 diff hist 0‎ Analysing NGS ‎No edit summary
• 09:3409:34, 1 April 2024 diff hist +917‎ Analysing NGS ‎No edit summary

26 March 2024

• 00:3400:34, 26 March 2024 diff hist +219‎ Orgenizing the data from the LCMS mechine ‎No edit summary current
• 00:3100:31, 26 March 2024 diff hist +191‎ N Orgenizing the data from the LCMS mechine ‎ Created page with "* In order to organize the data for further analysis , we use script in R * Please go over the presentations File:presentation1.ppt, File:presentation2.ppt, File:presentation2.ppt"
• 00:2800:28, 26 March 2024 diff hist +48‎ Laboratory for insect nutrition and metabolomics ‎No edit summary current
• 00:1900:19, 26 March 2024 diff hist 0‎ N File:Standard curve.png ‎No edit summary current
• 00:1700:17, 26 March 2024 diff hist +465‎ Designing and testing the qPCR primers ‎No edit summary current
• 00:1400:14, 26 March 2024 diff hist +768‎ Designing and testing the qPCR primers ‎No edit summary
• 00:1100:11, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:1000:10, 26 March 2024 diff hist 0‎ N File:Melting curve.png ‎No edit summary current
• 00:0900:09, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary Tag: Manual revert
• 00:0800:08, 26 March 2024 diff hist 0‎ N File:Melting curve2.png ‎No edit summary current
• 00:0700:07, 26 March 2024 diff hist −1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:0600:06, 26 March 2024 diff hist +1‎ Designing and testing the qPCR primers ‎No edit summary
• 00:0500:05, 26 March 2024 diff hist 0‎ N File:Meting curve.png ‎No edit summary current
• 00:0300:03, 26 March 2024 diff hist −1‎ Designing and testing the qPCR primers ‎No edit summary

25 March 2024

• 23:2223:22, 25 March 2024 diff hist 0‎ Designing and testing the qPCR primers ‎No edit summary
• 23:2123:21, 25 March 2024 diff hist +29‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1923:19, 25 March 2024 diff hist 0‎ N File:Melting curve.pdf ‎No edit summary current
• 23:1923:19, 25 March 2024 diff hist +20‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1523:15, 25 March 2024 diff hist −6‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1523:15, 25 March 2024 diff hist +1,059‎ Designing and testing the qPCR primers ‎No edit summary
• 23:1323:13, 25 March 2024 diff hist +872‎ Designing and testing the qPCR primers ‎No edit summary
• 11:0811:08, 25 March 2024 diff hist +80‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 11:0611:06, 25 March 2024 diff hist +303‎ Designing and testing the qPCR primers ‎No edit summary
• 11:0311:03, 25 March 2024 diff hist 0‎ Designing and testing the qPCR primers ‎No edit summary
• 11:0211:02, 25 March 2024 diff hist +1,130‎ N Designing and testing the qPCR primers ‎ Created page with "# For additional information [https://www.dropbox.com/scl/fi/070fhrrw6heimv1xxuxzq/real-time-pcr-handbook-life-technologies-update-flr.pdf?rlkey=eddim7471x9l8uqww1234yg6w&dl=0] ===House keeping genes=== # When checking gene expressed in the BSF it is important to use housekeeping gene as a reference. # Housekeeping genes, also known as reference genes, are a set of genes that are constitutively expressed in cells and are essential for basic cellular functions. # T..."
• 10:5610:56, 25 March 2024 diff hist +91‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 10:5110:51, 25 March 2024 diff hist +3‎ CDNA synthesis ‎No edit summary current
• 10:5010:50, 25 March 2024 diff hist +141‎ CDNA synthesis ‎No edit summary
• 10:4910:49, 25 March 2024 diff hist +171‎ CDNA synthesis ‎No edit summary
• 10:4410:44, 25 March 2024 diff hist −4‎ CDNA synthesis ‎No edit summary
• 10:4410:44, 25 March 2024 diff hist +40‎ CDNA synthesis ‎No edit summary
• 10:3510:35, 25 March 2024 diff hist +248‎ CDNA synthesis ‎No edit summary

4 March 2024

• 00:3200:32, 4 March 2024 diff hist +11‎ Sample preparation for LCMS ‎No edit summary current

28 January 2024

• 09:0009:00, 28 January 2024 diff hist +6‎ Checking the RNA integrity on gel electrophoresis ‎No edit summary current
• 08:5708:57, 28 January 2024 diff hist +2‎ Checking the RNA integrity on gel electrophoresis ‎No edit summary
• 08:5508:55, 28 January 2024 diff hist +18‎ Checking the RNA integrity on gel electrophoresis ‎No edit summary
• 08:5408:54, 28 January 2024 diff hist 0‎ N File:RNA bands.jpeg ‎No edit summary current
• 08:5308:53, 28 January 2024 diff hist +951‎ Checking the RNA integrity on gel electrophoresis ‎No edit summary
• 08:5008:50, 28 January 2024 diff hist +728‎ N Checking the RNA integrity on gel electrophoresis ‎ Created page with "===Preparation of the gel=== * Prepare a 1.5-2% gel electrophoresis. * For example, for a small gel, add 0.53 g agarose and 35 ml TBE/TAE to an Erlenmeyer flask. * Microwave for 30-45 seconds, stop and swirl, and then continue towards a boil until the agarose is completely dissolved. * Let the agarose solution cool down and add 3 µl of 'redsafe' (the amount depends on the 'redsafe' company). * Pour the agarose into a gel tray with the well comb in place. * Use the s..."
• 08:4108:41, 28 January 2024 diff hist +56‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 08:3908:39, 28 January 2024 diff hist +786‎ N How to interpret nano-drop results ‎ Created page with "===260/280 Ratio=== * The ratio of absorbance at 260 nm and 280 nm is used to assess the purity of DNA and RNA. * A ratio of ~2.0 is generally accepted as 'pure' for RNA. * If the ratio is appreciably lower in either case, it may indicate the presence of protein, phenol, or other contaminants that absorb strongly at or near 280 nm. ===260/230 Ratio=== *This ratio is used as a secondary measure of nucleic acid purity. * Expected 260/230 values are commonly in the ra..." current
• 08:3608:36, 28 January 2024 diff hist −3‎ Laboratory for insect nutrition and metabolomics ‎No edit summary
• 08:3308:33, 28 January 2024 diff hist +19‎ How to interoperate nano-drop results ‎No edit summary current

19 December 2023

• 04:3604:36, 19 December 2023 diff hist +317‎ N Analysis LCMS results ‎ Created page with "* Workflow for analysis using MetaboAnalyst [https://www.dropbox.com/scl/fi/7tcri1zvj5zlbaf5u2xjr/Metaboanalyst-workflow.docx?rlkey=vf2b9771nic2dgmjnnsx70ymm&dl=0] * Information about MetaboAnalyst [https://www.dropbox.com/scl/fi/w8ldsd44jod8ahbxksftc/MEtaboanalyst-5.pdf?rlkey=jbhtvlxy2fp22msfcpgxpjfmz&dl=0]" current
• 04:3204:32, 19 December 2023 diff hist +28‎ Laboratory for insect nutrition and metabolomics ‎No edit summary