Shreffler:Notebook/Staff Meetings/2010/08/24

ShreffLab Staff Meetings

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*Wayne G. Shreffler 10:00, 24 August 2010 (EDT): Agenda Items EoE EDN ELISAs Sarita has ordered a kit to try? and will initially evaluate with stds from company? -- will ship on 9/3 Conference call with Mirna on Friday to discuss possible collaboration and availability of samples for validation has some samples to send us Component proposal -- WS still needs to work on Basophil Review data from last week responder, good IL-3 enhancement; detectable IL-13 with dose response at 6 hours and detectable at 18 hours, though maybe predominant IL-3 effect Review ALK data responder -- good responses at 1:1; expected results -- when will we get more mAb? Histamine data we have not seen more data send sups from this week Next experiments Repeat isolated basophil experiments this week Allergen Adjuvant 2D done by Galina confirms pI ~10 Hazmik has gels and will run this week needs plant protein in fasta Foreskin DC flow data unable to detect aldefluor -- surface marker staining is weak prob due to staining in large vol with substrate buffer discussed alternatives, will first try staining per routine following aldefluor incubation, then may try sorting prior to culture or sorting after for qPCR PM Baso no samples last week JAX quote for peptide arrays WS has to do train Stephanie for project role Stephanie almost self-sufficient now Carboplatin possible sample Wed U19 Set up PBMC last week to milk allergen, sort on Fri set up another this week contact Josh about buffer Other Discussion Hood certification/ CO2 tanks will take over cost of that room Things To Do Write up component proposal (Marcella and Wayne) obtain quote for peptide arrays send Bert U19 slides