Shreffler:Notebook/Staff Meetings/2010/08/17

ShreffLab Staff Meetings

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*Wayne G. Shreffler 09:55, 17 August 2010 (EDT): Agenda Items EoE EDN ELISAs Sarita has ordered a kit to try? and will initially evaluate with stds from company? Conference call with Mirna on Friday to discuss possible collaboration and availability of samples for validation Component proposal -- WS still needs to work on Basophil Review data from last week non-responder -- x2 will implement a 'pre-screen' protocol to avoid basophil isolation and Derp2 specific sensitization with bad donors will run sups for cytokine (CBA) from first 2 exps presuming responder sample obtained this week Review ALK data -- non-responder -- will not save sups Histamine data Background is ~15% -- why so high? donor is non-responder Next experiments Repeat with buffy's this week Allergen Adjuvant 2D done by Galina confirms pI ~10 Bert will arrange to have gel sent to Hazmik Foreskin DC flow data -- looked good Thu cells to characterize, Fri Aldefluor Flow Thu 5-7p mAb -- how will we approach PM Baso no samples last week JAX quote for peptide arrays WS has to do train Stephanie for project role Stephanie almost self-sufficient now Carboplatin no samples Isabelle's project -- multi-lingual Action plan available in Spanish, will get Portuguese version Has 30 pages of recipes compiled U19 Set up PBMC this week to milk allergen Bert will try to identify allergen source and obtain endotoxin kit Other Discussion Hood certification/ CO2 tanks will take over cost of that room Things To Do Write up component proposal (Marcella and Wayne) obtain quote for peptide arrays send Bert U19 slides