Jordan T. Detamore Week 2

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Contents

Purpose

The purpose of this assignment was to explore the genetics of different color flowers in order to determine what alleles were present. This data was then used to create a pure-breeding purple flower.

Methods/Results

Part 1

  • The given flowers were Green-1, Green-2, White(W), and Red(R).
  • These flowers were crossed with each other giving new colors: Blue, Yellow, Orange, Purple, Black
  • The colors were self-crossed to determine what true breeding flowers could be created
    • Initial Green-1 and White were true-breeding and a true breeding Red, Blue, and Yellow were discovered by self-crossing
  • By crossing all the true breeding flowers, dominance was determined
    • Green is dominant to white, blue and yellow
    • White is recessive to Green, Yellow, Blue, and Red
    • Red and Yellow have incomplete dominance producing orange
    • Blue and Yellow have incomplete dominance producing green
    • Red and Blue have incomplete dominance producing purple
  • Since it was discovered that Red and Blue produce Purple and also that a true breeding Purple could not be created without mutation, the True Breeding Blue and Red were mutated
    • Once a Purple flower was created by mutating either the Red or Blue true breeding flower, the Purple flower was self-crossed until a true breeding Purple flower was created

Data Tables

Allele Color
G Green
R Red
W White
Y Yellow
B Blue
Genotype Phenotype
WB Blue
WG Green
WR Red
WY Yellow
WW White
BG Green
BR Purple
BY Green
BB Blue
GR Black
GY Green
GG Green
RY Orange
RR Red
YY Yellow
  • Genotype of Starting Strains
    • Green-1: GG
    • Green-2: BY
    • Red: WR
    • White: WW

Part II

  • A mutant Orange flower was created by mutating a true-breeding Yellow flower

image:Mutant Orange Trey.png

  • The difference in Amino Acid structure between the Yellow flower and the Orange flower were observed
    • The comparison shows differences many differences in Amino Acid structure

Image:AA sequence.png

  • The difference in DNA sequence between the Yellow flower and Orange flower was also observed
    • The comparison shows a difference in the 46th position

Image:DNA sequences.png

Data & Files

Scientific Conclusion

The purpose was to manipulate the genetics of flowers using Aipotu in order to learn about the genetics of the flowers. From this information, we were challenged to make a true-breeding Purple flower. We were able to do that and manipulate the genetics of other flowers to fulfill the task.

Acknowledgements

  • I worked with my lab partners Zachary T. Goldstein and Matthew Allegretti in class on this assignment
  • I used Zachary T. Goldstein's wiki syntax in order to create the tables used in Part I
  • I received instructions and guidance on how to complete this assignment from Dr. Dahlquist's Bioinformatics Lab
  • While I worked with the people noted above, this individual journal entry was completed by me and not copied from another source.
  • Jordan T. Detamore 16:23, 13 September 2016 (EDT):

References


Useful links

Introductory tutorial

OpenWetWare help pages

Bioinformatics Lab Home Page

Kam D. Dahlquist

Jordan T. Detamore

LMU Seaver College of Science and Engineering

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Jordan T. Detamore Week 2

Jordan T. Detamore Week 3

Jordan T. Detamore Week 4

Jordan T. Detamore Week 5

Jordan T. Detamore Week 6

Jordan T. Detamore Week 8

Jordan T. Detamore Week 9

Jordan T. Detamore Week 10

Jordan T. Detamore Week 11

Jordan T. Detamore Week 12

Jordan T. Detamore Week 13

Jordan T. Detamore Week 14

Jordan T. Detamore Week 15

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