IGEM:Stanford/2009/Project Homeostasis/Experimental Archives

System Overviews

• Detailed Summary of Homeostasis Project

• Link to googledocs image: Device Level Diagram

• Image of System and Genes Created by Mary and Leon

• Image of System and Genes Created by Mary and Leon_version II

• Detailed Construction Plan

• Detailed Construction Plan (Updated)

• PDF of 4 Plasmid Designs

• PDF of Overview of Plasmids and System

• Pwpt of Combined Plasmids and Systems PDFs

Inflammation Device

Goal: To design the device that monitors and responds to inflammation.

• a. Input: a promoter that monitors inflammation by detecting levels of Th17-derived markers.
• b. Output: a gene that responds to inflammation by inhibiting the Th17 response and enhancing the Treg lineage.
• 

  

  Anti-Inflammatory Device (Developed So Far)

Device 1

Immunosuppression Device

Goal: To design the device that monitors and responds to immunosuppression.

• c. Input: a promoter that monitors immunosuppression by detecting levels of Treg-derived markers.
• d. Output: a gene that responds to Treg-driven immunosuppression by inhibiting the Treg response and enhancing the Th17 lineage.
• 

  

  Immunosuppression Device (Developed So Far)

Device 2

Discussion

• E.Coli Genome: http://ecocyc.org/

Paper Pages

• PLoS. Role of IL-6 in mice.

• Review of SoxR/SoxS

• E.Coli Export

Primer Design

• Guidelines and Helpful Websites
• Original Primers

Gene Sequences and Orders

• Link to googledocs: Sources of Genes and Sequences

• • Sources of Genes and Sequences (googledocs is better)

• Gene Sequences w/ Highlighted RE Sites Document

• Sequences to be De Novo Synthesized

• Stanford IGEM's 4 de novo synthesized sequences

Trp Repressor Analogue

• 5-methyltryptophan [1]
• Trp Notes From Yanofsky Meeting 1

Parts

• Link to Spreadsheet on googledocs to Edit: BioBrick parts and Assay Ideas

• Tentative List of Parts for Registry Image:Parts.doc