Haynes Lab:Notebook/CRISPR Editing/2015/03/27

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03/27/2015

Ran on gel, purified, and set up digests of nested PCR products from yesterday for g034, unt and g048

Cell type Treatment rep F primer R primer 260 280 260/280 ng/µL ul for 200ng ul for 400ng ul water ul buffer ul enzyme
Luc14g0341P163P2150.0540.0291.83454.0547.409.6021
Luc14g0342P163P2150.0530.0281.86952.5377.619.3921
Luc14g0343P163P2150.0560.031.83555.657.199.8121
Gal4-EED +doxg0341P163P2150.0550.0311.854.9017.299.7121
Gal4-EED +doxg0342P163P2150.0590.0321.85159.3656.7410.2621
Gal4-EED +doxg0343P163P2150.0520.0271.88251.5587.769.2421
Luc14Unt1P163P2150.0570.0321.81757.3566.9710.0321
Luc14Unt2P163P2150.0630.0341.87263.426.3110.6921
Luc14Unt3P163P2150.0520.0271.88951.5477.769.2421
Gal4-EED +doxUnt1P163P2150.0460.0251.83145.5668.788.2221
Gal4-EED +doxUnt2P163P2150.0490.0281.71948.8798.188.8221
Gal4-EED +doxUnt3P163P2150.0480.0271.78947.5468.418.5921
Luc14g0481P213P2140.0440.0241.80543.8854.5612.4421
Luc14g0482P213P2140.0430.0231.88642.7984.6712.3321
Luc14g0483P213P2140.0360.021.83736.4915.4811.5221
Gal4-EED +doxg0481P213P2140.0330.0191.77233.3815.9911.0121
Gal4-EED +doxg0482P213P2140.0420.0251.70742.3414.7212.2821
Gal4-EED +doxg0483P213P2140.0360.0211.73536.0425.5511.4521



gDNA extraction

Cell type Plasmid 1 Plasmid 2 260 280 260/280 ng/µL ul for 200ng ul water to 4
Luc14pMax GFP 6.2g0340.0830.042.05282.6012.421.58
Luc14KAH228pMax GFP 6.20.0910.0461.99391.442.191.81
Luc14KAH228g0340.130.0671.934129.8261.542.46
Gal4-EEDpMax GFP 6.2g0340.1340.0691.96134.4941.492.51
Gal4-EEDKAH228pMax GFP 6.20.150.0771.95150.2491.332.67
Gal4-EEDKAH228g0340.130.0691.886130.0941.542.46

phusion PCR with P197/198


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