Haynes Lab:Notebook/CRISPR Editing/2015/05/01

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05/01/2015 Cell type gRNA 260 280 260/280 ng/µL Luc14 g051 0.213 0.107 1.993 213.13 Luc14 g051 0.16 0.079 2.012 159.928 Luc14 g052 0.196 0.097 2.011 195.786 Luc14 g052 0.189 0.096 1.974 188.894 Luc14 g053 0.305 0.152 1.997 304.55 Luc14 g053 0.121 0.063 1.938 121.418 Luc14 g054 0.168 0.084 2.005 168.386 Luc14 g054 0.138 0.068 2.02 137.513 Luc14 g055 0.17 0.086 1.97 170.326 Luc14 g055 0.201 0.102 1.977 200.799 Luc14 g056 0.092 0.047 1.96 91.739 Luc14 g056 0.176 0.089 1.986 176.135 Cell type gRNA ug DNA ul lipo 260 280 260/280 ng/µL Gal4-EED g034 0.5 3 0.081 0.043 1.877 81.058 Gal4-EED g034 0.5 3 0.087 0.049 1.753 86.697 Gal4-EED g034 0.5 5 0.024 0.013 1.876 23.89 Gal4-EED g034 0.5 5 0.025 0.013 1.975 24.767 Gal4-EED g034 1.0 3 0.085 0.046 1.842 85.32 Gal4-EED g034 1.0 3 0.07 0.038 1.849 70.244 Gal4-EED g034 1.0 5 0.013 0.009 1.5 13.387 Gal4-EED g034 1.0 5 0.023 0.013 1.754 23.213