Haynes Lab:Notebook/CRISPR Editing/2015/03/26

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03/26/2015

Redoing PCR with P197/198 of gDNA from g034 and g048 treated cells:

Cell type Treatment Rep 260 280 260/280 ng/┬ÁL ul for 200ng ul water
Luc14g03410.0520.0252.06451.823.860.14
Luc14g03420.0970.0491.99396.7572.071.93
Luc14g03430.0810.042.01380.7622.481.52
Gal4-EED +doxg03410.0880.0461.93888.4522.261.74
Gal4-EED +doxg03420.1150.0581.974114.7091.742.26
Gal4-EED +doxg03430.0650.0312.10564.9453.080.92
Luc14g04810.1070.0532.028106.5041.882.12
Luc14g04820.1150.0581.961114.6111.752.25
Luc14g04830.0490.0242.0349.4564.04-0.04
Gal4-EED +doxg04810.0920.046292.3612.171.83
Gal4-EED +doxg04820.0890.0442.01989.0412.251.75
Gal4-EED +doxg04830.0620.0321.95261.5963.250.75



Annealing g044 and unt P213/214 PCR products for Surveyor according to surveyor protocol

will rerun g025 and unt P173/215 surveyor treated samples on biodesign bioanalyzer



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