Biomod/2014/NTU/Equipment

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Agarose Gel Electrophoresis

Gel electrophoresis is a method used to separate DNA structures by size and charge. We also use gel electrophoresis to verify the presence of aggregation of the nanojellyfish origami.

Atomic Force Microscopy

AFM(Atomic Force Microscopy) is a type of scanning probe microscopy with high-resolution on the order of nanometer. It works by oscillating the cantilever to detect the perturbations of the sample’s surface. We used this machine to confirm the structure of our DNA-origami.

Thermal Cycler

Thermal cycler is used to control the reaction temperature for our nanojellyfish to assemble into the designed structures correctly.

Centrifuge

Centrifuge is a method to purify our samples and primers. It works by the sedimentation principle, causing denser substances to separate out along the radial direction.

<img src="http://openwetware.org/images/e/e9/TEM.jpg"/>

TEM

In order to examine the structure and function of our nanojellyfish origami, we used the transmission electron microscopy (TEM). This machine works by transmitting a beam of electrons through the specimen. This specimen is able to interact with the electrons and form an image. In this case, the nanojellyfish are detected as hollow circles on a computer screen.

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