BME100 f2015:Group8 8amL2

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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
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Contents

OUR TEAM

Name: Riiley Barnett
Name: Riiley Barnett
Name: Manuela Hiche
Name: Manuela Hiche
Name: Luis Montero
Name: Luis Montero
Name: Cody Wong
Name: Cody Wong
Name: Nicholas Grant
Name: Nicholas Grant
Name: Chris A Pina 1
Name: Chris A Pina 1

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1
Dosage of LPS in Humans

Experiment 2
Dosage of LPS in Rats




Results

Experiment 1

Using 40 subjects we tested if LPS treatment increased inflammotin protein levels in the blood. After analyzing the data, a positive correlation is shown between the LPS dosage given to humans and the levels of inflammotin present in the body. As the dosage given increased, the average inflammotin protein levels increased (see graph). Using ANOVA, we were able to determine the p value of 1.4X 10^-16. All dosages had a p value of less than 0.05 which shows that there is a statistical difference with 95% confidence or greater.


Experiment 2

Based on the data acquired in experiment 2, there is no correlation between the LPS dosage given to rats and the levels of inflammotin present in the body. This can be seen using the pearson correlation comparing the control group of 0mg to the group given 10mg. The correlation was -.57, showing that no correlation can be determined.




Analysis

Experiment 1

There was a correlation between the LPS dosage given to humans and the levels of Inflammotin present in the body. As the dosage of LPS increased, the Inflammotin in the body increased as well.

Experiment 2

Unlike humans, rats always have the protein inflammotin present in their bodies. The addition of this experimental drug does not seem to impact the levels of inflammotin present in rats.




Summary/Discussion

In conclusion we have found a direct correlation between lipopolysaccharide (LPS) and increase levels of the protein inflammotin in humans. The same did not apply for rats. Rats have shown a consistent presence of the protein inflammotin in their bodies even with no LPS given. In future experiments it would be beneficial to include more rats in the experiment to lower the standard deviation. Even with this modification to the experiment, if there is no correlation between the two test subjects, it can be inferred that rats are not a good non-human test subject for this experiment.

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