BME100 f2015:Projects4

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Lab 4: “Experimental Design”

  • Content from PCR Lab A and Lab B
    • Design a protocol
    • Research and development - the underlying technology
    • SNP information & primer design

HW: Lab Report 4 (due 10/20/15 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
15 points – Protocol: Materials, PCR reaction sample list, DNA sample set-up procedure, OpenPCR program
15 points – Research & Development: function of components, PCR steps, base-pairing
10 points - SNP information & primer design
50 points – Peer Assessment

Rubric

    EXCELLENT ACCEPTABLE WEAK*
OVERALL LAYOUTOUR TEAM section5 pts = Original & non-offensive images; real names3 pts = More than half original images & information1 pt = All default green & white avatars; default text
Grammar & style5 pts = Composition & layout perfect throughout3 pts = Few spelling errors; images too big or small1 pt = Many errors
PROTOCOL Materials & PCR Reaction Sample List5 pts = Materials and all 8 samples & tube labels shown in Wiki table format3 pts = Formatting mistakes (missing table) 1 pts = Tube labels missing
DNA Sample Set-up Procedure5 pts = Consistent with the worksheet; phrased in student's own words3 pts = Copied text from worksheet1 pts = Missing one: PCR reaction mix, DNA/ primer mix
OpenPCR program5 pts = Complete text list or screen capture from OpenPCR software3 pts = Copy-pasted text from worksheet; no original comments from students1 pts = Missing one or more: initial step, denature, anneal, extend, final step, final hold
RESEARCH & DEVELOPMENTPCR - The underlying technology15 pts = Complete: function of components, PCR steps, base pairing10 pts = Mising one part; very short overall5 pts = Missing two or more parts
BONUS (5 points) - original digital or drawn illustration of PCR5 pts = Composed from scratch (not from the internet)4 pts = Images from the web with good captions/ labels3 pts = Images from the web, lacking text explanation
SNP INFO. & PRIMER DESIGNBackground5 pts = Well-written full sentences. 3 pts = Unclear, short (< 5 sentences), mostly phrases/ fragments 1 pt = Unclear, extremely short
Primer design & testing5 pts = Short summary & image of web tool results3 pts = Missing summary1 pt = Missing image or both image & summary

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points


Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plagiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.



Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.

Group1_8amL4 Group2_8amL4 Group3_8amL4 Group4_8amL4 Group5_8amL4
Group6_8amL4 Group7_8amL4 Group8_8amL4 Group9_8amL4 Group10_8amL4
Group11_8amL4 Group12_8amL4 Group13_8amL4 Group14_8amL4 Group15_8amL4
Group16_8amL4 Group17_8amL4
 
Group1_1030amL4 Group2_1030amL4 Group3_1030amL4 Group4_1030amL4 Group5_1030amL4
Group6_1030amL4 Group7_1030amL4 Group8_1030amL4 Group9_1030amL4 Group10_1030amL4
Group11_1030amL4 Group12_1030amL4 Group13_1030amL4 Group14_1030amL4 Group15_1030amL4
Group16_1030amL4 Group17_1030amL4
 
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