BME100 f2015:Projects5

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Lab 5: “PCR and Fluorescence Detection of DNA”

  • Content from PCR Lab C and Lab D
    • PCR Reactions
    • Procedure
    • Data Analysis

HW: Lab Report 5 (due 11/03/15 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
10 points - Lab C content - PCR reaction report
15 points – Lab D content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter
15 points – Lab D content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary
50 points – Peer Assessment

Rubric

    EXCELLENT ACCEPTABLE WEAK*
OVERALL LAYOUTOUR TEAM section5 pts = Original & non-offensive images; real names3 pts = More than half original images & information1 pt = All default green & white avatars; default text
Grammar & style5 pts = Composition & layout perfect throughout3 pts = Few spelling errors; images too big or small1 pt = Many errors
PCR REACTION REPORT Summary10 pts = Complete description, good text formatting5 pts = Poor composition1 pt = Missing (only default text)
FLUORIMETER PROCEDURECamera settings5 pts = Type of smartphone; settings where applicable, or "N/A"3 pt = Brand name only; vague1 pts = Missing all info
Camera set-up 5 pts = Clear description; distance btwn. camera & drop included 3 pts = Missing one part of the information 2 pts = Only one sentence, missing distance
Placing samples onto the fluorimeter5 pts = Steps are thorough, clear, and easy to follow3 pts = Run-on paragraphs1 pt = Instructions are vague/ incomplete
DATA COLLECTION & ANALYSIS Callibration: 3 drop images, data table, 2 plots with best fit lines7 pts = Complete5 pts = Missing one part, somewhat unorganized3 pts = Missing two or more parts, very unorganized
PCR results: 2 drop images, data table, final results8 pts = Complete6 pts = Missing one part, somewhat unorganized4 pts = Missing two or more parts, very unorganized

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points


Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.



Look for your Group's lab report link in the list of links on the right. If it is RED instead of blue, DO NOT CLICK ON IT!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.

Group1_8amL5 Group2_8amL5 Group3_8amL5 Group4_8amL5 Group5_8amL5
Group6_8amL5 Group7_8amL5 Group8_8amL5 Group9_8amL5 Group10_8amL5
Group11_8amL5 Group12_8amL5 Group13_8amL5 Group14_8amL5 Group15_8amL5
Group16_8amL5 Group17_8amL5
 
Group1_1030amL5 Group2_1030amL5 Group3_1030amL5 Group4_1030amL5 Group5_1030amL5
Group6_1030amL5 Group7_1030amL5 Group8_1030amL5 Group9_1030amL5 Group10_1030amL5
Group11_1030amL5 Group12_1030amL5 Group13_1030amL5 Group14_1030amL5 Group15_1030amL5
Group16_1030amL5 Group17_1030amL5
 
GroupTA_L5
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