User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Data==
==Data==
<u>Sample Prep</u>
<u>Stock solution for class</u>
# AgNO3
## 28.13 mg AgNO3
## to 10.0 mL in water
## 169.87 g/mol
## 16.6 mM
 
<u>Myoglobin Sample Prep</u>
# 250 μM AuCl3, 12.5 μM myoglobin, pH 4
# 250 μM AuCl3, 12.5 μM myoglobin, pH 4
## 176 μL 4.27 mM AuCl3
## 176 μL 4.27 mM AuCl3
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## 300 μL 1mM HCl
## 300 μL 1mM HCl
## 2290 μL water
## 2290 μL water
<u>UV-Vis of sample before and after measurement</u>
[[Image:20160928 mrh MyoglobinAuNPUVVis.png]]
<u>Fluorescence data</u>
# Acquisition information
## Excitation: 290 nm, 10 nm slit width
## Emission: 310-540 nm, 10 nm slit width
## 100 nm/minute
## 61 measurements, 180 second delay between measurements
[[Image:20160928 mrh MbAuNP IntegratedI.png]]
[[Image:20160928 mrh MbAuNP EmissionMax.png]]
[[Image:20160928 mrh MbAuNP selectedscans1.png]]
[[Image:20160928 mrh MbAuNP selectedscans2.png]]
[[Image:20160928 mrh MbAuNP selectedscans3.png]]


==Notes==
==Notes==
This area is for any observations or conclusions that you would like to note.


[[User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/09/28| Anneliese Faustino]]
[[User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/28|Hannah Harvey]]
[[User:Sara K. Mattson/Notebook/Chemistry 671/2016/09/27|Sara Mattson]]
[[User:Patrick Hampson/Notebook/chem471/2016/09/28|Patrick Hampson]]


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
[[User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/28 | Sarah Burkhard]]


[[Category:Course]]
[[Category:Course]]

Latest revision as of 02:03, 27 September 2017

Biomaterials Design Lab Main project page
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Objective

Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solution for class

  1. AgNO3
    1. 28.13 mg AgNO3
    2. to 10.0 mL in water
    3. 169.87 g/mol
    4. 16.6 mM

Myoglobin Sample Prep

  1. 250 μM AuCl3, 12.5 μM myoglobin, pH 4
    1. 176 μL 4.27 mM AuCl3
    2. 234 μL 160 μM myoglobin
    3. 300 μL 1mM HCl
    4. 2290 μL water


UV-Vis of sample before and after measurement

Fluorescence data

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Notes

Anneliese Faustino

Hannah Harvey

Sara Mattson

Patrick Hampson

Sarah Burkhard




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